CD52 Molecule (CD52) antibody (PE)

Antigen: CD52 Molecule (CD52)

Synonyms: CDW52, B7, MB7, CLS1, B7-Ag, AI463198, CAMPATH-1, CD52

Clonality: Monoclonal (HI186)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN125720

Additional Information

Alternative name: CD52

Immunogen: Human tonsil

Isotype: IgG2b

Clone: HI186

Description: CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptidewhich is present at high levels on lymphocytes, macrophages, epithelial cells of malereproductive tract and mature sperm. Its 12-amino acid beckbone carries a complexN-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well asthe GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where itis released by epithelial cells. Although CD52 is not an essential T-cell costimulator, itstriggering results in activation of normal human T cells. CD52 is a very good target forantibody/complement-mediated cell lysis.

Specificity: The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa glycoprotein containing alarge N-linked carbohydrate moiety, mature CD52 molecule is actually much smaller(approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes,monocytes/macrophages and in male reproductive tract.HLDA VI, WS Code BP 523HLDA VI, WS Code T 6T-057

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only