Integrin, alpha L (Antigen CD11A (p180), Lymphocyte Function-Associated Antigen 1; alpha Polypeptide) (ITGAL) antibody

Antigen: Integrin, alpha L (Antigen CD11A (p180), Lymphocyte Function-Associated Antigen 1; alpha Polypeptide) (ITGAL)

Synonyms: CD11A, LFA-1, LFA1A, Cd11a, Ly-15, Ly-21, ITGAL, CD11a

Clonality: Monoclonal (MEM-83)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Catalog no.: ABIN125722

Additional Information

Alternative name: CD11a

Immunogen: Human peripheral blood lymphocytes

Format: Azide Free

Isotype: IgG1

Clone: MEM-83

Description: CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1(LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex.LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation.Cell stimulation by chemokines or other signals leads to induction the high-affinityconformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesionmolecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells andin their tissue-specific settlement, but participates also in control of cell differentiation andproliferation and of T-cell effector functions. Blocking of LFA-1 function by specificantibodies or small molecules has become an important therapeutic approach in treatmentof multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab(Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding ofcholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulationand increase in lymphocytic cholinergic activity.

Specificity: The antibody MEM-83 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDatype I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes,macrophages, neutrophils, basophils and eosinophils.HLDA IV, WS Code N 211

Application Details

Application Notes: Functional Application: The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an invitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped tothe "I" domain of the LFA-1 alpha subunit. The studies have therefore identified a novelLFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in theregulation of LFA-1 binding to ICAM-1. Immunoprecipitation

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Landis, McDowall, Holness et al.: "Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3." in: The Journal of cell biology, Vol. 126, Issue 2, pp. 529-37, 1994 (PubMed).

Tran, Glass, Uzel et al.: "Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 5, pp. 2929-38, 2009 (PubMed).