CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (Chain epsilon) antibody

Details for Product No. ABIN125723
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Antigen
Synonyms cd3e, CD3E, t3e, tcre, AI504783, CD3, CD3epsilon, T3e, T3E, TCRE
Epitope
Chain epsilon
(27), (8), (7), (7), (6), (6), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(278), (171), (36), (31), (6), (1), (1), (1), (1), (1)
Host
Mouse
(165), (113), (106), (50), (16), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(55), (45), (44), (24), (10), (10), (9), (7), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application
Functional Studies (Func), Immunoprecipitation (IP), Flow Cytometry (FACS)
(343), (116), (106), (95), (55), (37), (35), (31), (30), (20), (18), (3), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN125723
304.92 $
Plus shipping costs $45.00

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Immunogen Human peripheral blood lymphocytes.
Clone MEM-92
Isotype IgM
Specificity The antibody MEM-92 reacts with epsilon chain of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.HLDA IV., WS Code T 97
Purification Purified from ascites by thiophilic adsorption-affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Alternative Name CD3E
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T-cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-82 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells
Application Notes Flow Cytometry: Recommended dilution: 2-10 µg/mL

Functional Application: The antibody MEM-92 in solution induces early responses of T cell activation (tyrosine phosphorylation, calcium elevation, Erk activation and expression of activation antigens), but it is unable to induce T cell proliferation.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
General Stefanovua, Saville, Peters et al.: "HIV infection--induced posttranslational modification of T cell signaling molecules associated with disease progression." in: The Journal of clinical investigation, Vol. 98, Issue 6, pp. 1290-7, 1996 (PubMed).

Brdicka, Imrich, Angelisová et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Batista, Milluan, Mittelbrunn et al.: "Recruitment of transferrin receptor to immunological synapse in response to TCR engagement." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6709-14, 2004 (PubMed).

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