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Antibodies
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anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (PE)
HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (PE)
| Antigen |
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| Synonyms |
MHC-G |
| Clonality |
Monoclonal (87G) |
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Host
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Reactivity
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Conjugate
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Application
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Flow Cytometry (FACS) (68), ELISA (30), Western Blotting (WB) (29), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Immunoprecipitation (IP) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Enzyme Immunoassay (EIA) (12), Immunocytochemistry (ICC) (10), Immunofluorescence (IF) (8), Functional Studies (Func) (3), Immunohistochemistry (IHC) (2)
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| Catalog no. |
ABIN125728 |
| Quantity |
0.1 mg (0.1 mg/ml) |
| Price |
507.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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HLA-G
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Immunogen
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HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with andexpressing genes encoding HLA-G and human beta2-microglobulin.
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Isotype
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IgG2a
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Clone
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87G
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Specificity
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The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical)and it is expressed on the surface of trophoblast cells.The antibody 87G blocks interaction of HLA-G with inhibitory receptors. This antibody does not react with: Mouse, Rat .
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Application Notes
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The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1: 10.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
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Concentration
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0.1 mg/ml
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Buffer
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The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. Preparation: The purified antibody is conjugated with R-Phycoerythrin (PE)
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Storage
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Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
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Research Area
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Major Histocompatibility Complex (MHC)
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Restrictions
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For Research Use only
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Alternatives for antigen "HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG)", type "Antibodies"
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Hosts
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Mouse (99)
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Reactivities
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Human (99)
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Applications
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Flow Cytometry (FACS) (68), ELISA (30), Western Blotting (WB) (29), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Immunoprecipitation (IP) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Enzyme Immunoassay (EIA) (12), Immunocytochemistry (ICC) (10), Immunofluorescence (IF) (8), Functional Studies (Func) (3), Immunohistochemistry (IHC) (2)
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Conjugates
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Biotin (11), FITC (11), PE (9), Alexa Fluor 488 (7), APC (2), HRP (2)
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Alternatives 
