SPS antibody

Catalog No. ABIN5706929

Product Details

Target: SPS

Reactivity: Zea mays, Arabidopsis thaliana, Barley, Potato, Tomato, Wheat

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Predicted Reactivity: dicots including: Glycine max, Vicia faba,Solanum tuberosum, monocots including: Oryza sativa, trees: Populus balsamifera, Robinia pseudoacacia, moss: Physcomitrella patens

Characteristics: Expected / apparent Molecular Weight of the Antigene: 120 / 120-130 kDa (fragments of 30/90 kDa may be detected)

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from conserved region within plant SPS protein sequences, including Arabidopsis thaliana isoforms 1F Q94BT0, 2F, 3F and 4F. Oryza sativa Q67WN8, Solanum tuberosum Q43845

Application Details

Application Notes: Recommended Dilution 1 : 1000 - 1 : 5000 with standard ECL (WB).

Comment:

Peptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References

Yancey et al.: "Commentary: Omalizumab therapy for bullous pemphigoid by KK Yu, AB Crew, KAN Messingham, JA Fairley, DT Woodley. J Am Acad Dermatol 2014 Jun 20. pii: S0190-9622(14)01410-8. doi: ..." in: Dermatologic therapy, Vol. 28, Issue 1, pp. 3, (2015) (PubMed).

Whittaker, Martinelli, Farrant, Bochicchio, Vazzana: "Sucrose phosphate synthase activity and the co-ordination of carbon partitioning during sucrose and amino acid accumulation in desiccation-tolerant leaf material of the C4 resurrection plant Sporobolus stapfianus during dehydration." in: Journal of experimental botany, Vol. 58, Issue 13, pp. 3775-87, (2007) (PubMed).

Target Details

Target: SPS

Background: AGI Code: At5g20280
SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

Molecular Weight: expected: 120 kDa, apparent: 120-130 kDa (fragments of 30/90 kDa may be detected)

UniProt: Q94BT0, Q43845, Q67WN8