Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

PRDX6-PS2 antibody (C-Term)

PRDX6-PS2 Reactivity: Arabidopsis thaliana ELISA, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN125822
  • Target
    PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
    Binding Specificity
    C-Term
    Reactivity
    Arabidopsis thaliana
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    ELISA, Western Blotting (WB)
    Cross-Reactivity (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 25 / 20 kDa
    Purification
    serum
    Immunogen
    thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of AtGPX7 accession At4g31870 has been used for antibody production
  • Application Notes
    1: 5000 with alkaline phosphatase system (AP) (WB), 1:1, 1:2, 1:4, 1:8, 1: 16 (ELISA)
    Comment

    This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 μL of sterile water.
    Handling Advice
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Lepistö, Pakula, Toivola, Krieger-Liszkay, Vignols, Rintamäki: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).

    Kim, Jin, Yoon: "Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadione." in: Cell stress & chaperones, (2010) (PubMed).

  • Target
    PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
    Alternative Name
    GPX
    Background
    AGI Code: At4g31870
    GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.
    Molecular Weight
    expected: 25 kDa, apparent: 20 kDa
    UniProt
    Q9SZ54
You are here:
Support