ARGSVIL antibody

Antigen: ARGSVIL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN125917

Additional Information

Immunogen: The monoclonal antibody was raised against the N-terminal sequence ARGSVIL.

Format: Affinity-purified

Description: The monoclonal antibody was raised against the N-terminal sequence ARGSVIL and purified by affinity chromatography on protein A. Purified antibody in 100 µl phosphate buffered saline (20 mM Na-phosphate, pH 7.5, 150 mM NaCl) containing 0.01 % thimerosal.

Specificity: The antibody reacts with the N-terminal sequence ARGSVIL of aggrecan fragments produced by proteolysis at the aggrecanase site within the interglobular domain of aggrecan (aggrecan-IGD). It does not react to a significant extent with the sequence ARGSVIL contained within uncleaved polypeptide chains. ARGSVIL-peptides produced from recombinant aggrecan - IGD in the presence of aggrecanases bind strongly to the antibody both in Western Blots and in ELISA.

Application Details

Application Notes: Western-Blotting with ECL-Detection, suggested antibody dilution: 1: 2000

Storage: The antibody is stable until the expiration date given on the label date when stored at -70 °C. After thawing it can be kept at 2-8 °C for more than 3 months. Repeated freezing and thawing should be avoided.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Images

Western Blot of recombinant aggrecan-IGD and derived ARGSVIL-peptide with anti- N-terminal sequence GSVIL antibody. Lane 1: 100 ng uncleaved aggrecan-IGD Lane 2: 100 ng ARGSVIL-peptide Lane 3: 25 ng ARGSVIL-peptide The apparent Mr of uncleaved aggrecan-IGD in SDS-PAGE is 22 kDa.