Actin antibody

Antigen: Actin

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Clonality: Monoclonal (2G2)

Host: Mouse

Reactivity: Cow (Bovine), Chicken, Human, Rabbit, Xenopus laevis

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN125946

Additional Information

Alternative name: Actin (pan)

Immunogen: Profilin Actin complex from calf thymusEpitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA

Format: Supernatant

Isotype: IgM  (Matching secondary antibodies)

Clone: 2G2

Description: The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actinand myosin are constituents of many cell types and are involved in a myriad of cellularprocesses including locomotion, secretion, cytoplasmic streaming, phagocytosis andcytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresisand amino acid sequence analysis. Four of them represent the differentiation markers ofmuscle tissues and two are found in practically all cells.

Specificity: Specific for skeletal and non-muscle actin isoforms (Mr 43,000). Reactivities on Cultured Cell Lines (tested so far): PtK2, SR-NRK, NRK-49F, L6 cells, ratfibroblasts, NIH-3T3, mouse myoblast and myotube cells. Species: Human, Bovine, Rabbit, Chicken and Xenopus laevis. Others not tested.

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Application Details

Application Notes: Immunoblotting. Cytological material. Immunofluorescence Microscopy (Only after methanol fixation). Immunohistochemistry on Frozen and Paraffin Sections: Ready to use forimmunohistochemistry. Incubation Time: 1h at RT (extended with paraffin). With paraffin embedded sections,protease pretreatment is required prior to antibody application. When using antibody 2G2 in immunolocalization studies the presence of nuclear actincould be demonstrated e. g. in cultured cells after fixation with 4% paraformaldehyde. Immunostaining with cytoplasmic actin was only positive after fixation with methanol (cf. Gansior et al. , 1999). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Storage: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Publications

Publications: Gonsior, Platz, Buchmeier et al.: "Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody." in: Journal of cell science, Vol. 112 ( Pt 6), pp. 797-809, 1999 (PubMed).

Hofmann, Reichart, Ewald et al.: "Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin." in: The Journal of cell biology, Vol. 152, Issue 5, pp. 895-910, 2001 (PubMed).

Zhang, Buder, Burkhardt et al.: "Nuclear DNA helicase II/RNA helicase A binds to filamentous actin." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 843-53, 2002 (PubMed).

Peitsch, Hofmann, Endlich et al.: "Cell biological and biochemical characterization of drebrin complexes in mesangial cells and podocytes of renal glomeruli." in: Journal of the American Society of Nephrology : JASN, Vol. 14, Issue 6, pp. 1452-63, 2003 (PubMed).

Bettinger, Gilbert, Amberg: "Actin up in the nucleus." in: Nature reviews. Molecular cell biology, Vol. 5, Issue 5, pp. 410-5, 2004 (PubMed).