Basic Cytokeratin antibody (Biotin)

Details for Product No. ABIN125986
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Antigen
Reactivity
Amphibian, Cow (Bovine), Human, Rat (Rattus)
(6), (6), (5), (3), (3), (3), (2), (2), (2)
Host
Mouse
(6)
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
(1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(6), (5), (5), (4), (2), (1)
Pubmed 5 references available
Quantity 0.25 mL
Shipping to United States (Change)
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Catalog No. ABIN125986
478.50 $
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Immunogen Cytoskeletal proteins from cultured human MCF-7 cells
Clone Ks pan 1-8
Isotype IgG2a
Cross-Reactivity (Details) Species reactivity (tested):Human, Bovine, Rat, Amphibia.
Characteristics Molar Ratio: 5 mol biotin / 1 mol IgG
Purification Protein A affinity chromatography
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).
Application Notes Immunohistochemistry on frozen sections and cytological material: 10 - 20 μg/mL (dilutewit PBS), incubation time 1 h at RT. Flow cytometry. Cell sorting. Immunoblotting (Western). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.2 mg/mL
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Gould, Koukoulis, Jansson et al.: "Coexpression patterns of vimentin and glial filament protein with cytokeratins in the normal, hyperplastic, and neoplastic breast." in: The American journal of pathology, Vol. 137, Issue 5, pp. 1143-55, 1990 (PubMed).

Achtstätter, Fouquet, Rungger-Brändle et al.: "Cytokeratin filaments and desmosomes in the epithelioid cells of the perineurial and arachnoidal sheaths of some vertebrate species." in: Differentiation; research in biological diversity, Vol. 40, Issue 2, pp. 129-49, 1989 (PubMed).

General Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Heid, Moll, Franke: "Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles." in: Differentiation; research in biological diversity, Vol. 37, Issue 2, pp. 137-57, 1988 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

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