Basic Cytokeratin antibody (Biotin)

Details for Product No. ABIN125986
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Antigen
Reactivity
Amphibian, Cow (Bovine), Human, Rat (Rattus)
(6), (5), (5), (3), (3), (3), (2), (2), (2)
Host
Mouse
(6)
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
(1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS)
(6), (5), (4), (4), (2), (1)
Pubmed 5 references available
Catalog no. ABIN125986
Quantity 0.25 mL
Price
478.50 $   Plus shipping costs $45.00
Shipping to (Change)
Availability Will be delivered in 6 to 8 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Cytoskeletal proteins from cultured human MCF-7 cells
Clone Ks pan 1-8
Isotype IgG2a
Specificity Polypeptides reacting: Mr 52500 - Mr 68000 keratins (type II keratins K1 - K8, formerly also designated cytokeratins 1 - 8) of human epithelial cells. Tumors specifically detected: all epithelium-derived neoplasms. Reactivities on cultured cell lines (tested so far): MCF-7, RT112, HT-29, Detroit 562, RPMI2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK. This antibody represents an excellent marker for distinguishing carcinomas from non- epithelial tumors.
Characteristics Molar Ratio: 5 mol biotin / 1 mol IgG
Purification Protein A affinity chromatography
Application Notes Immunohistochemistry on frozen sections and cytological material: 10 - 20 µg/ml (dilutewit PBS), incubation time 1 h at RT. Flow cytometry. Cell sorting. Immunoblotting (Western). ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.2 mg/mL
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Gould, Koukoulis, Jansson et al.: "Coexpression patterns of vimentin and glial filament protein with cytokeratins in the normal, hyperplastic, and neoplastic breast." in: The American journal of pathology, Vol. 137, Issue 5, pp. 1143-55, 1990 (PubMed).

Achtstätter, Fouquet, Rungger-Brändle et al.: "Cytokeratin filaments and desmosomes in the epithelioid cells of the perineurial and arachnoidal sheaths of some vertebrate species." in: Differentiation; research in biological diversity, Vol. 40, Issue 2, pp. 129-49, 1989 (PubMed).

General Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Heid, Moll, Franke: "Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles." in: Differentiation; research in biological diversity, Vol. 37, Issue 2, pp. 137-57, 1988 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Hosts (6)
Reactivities (6), (5), (5), (3), (3), (3), (2), (2), (2)
Applications (6), (5), (4), (4), (2), (1)
Conjugates (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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