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Keratin Basic antibody

Reactivity: Human, Rat, Cow, Amphibian WB, IF, IHC (fro) Host: Mouse Monoclonal Ks pan 1-8 unconjugated
Catalog No. ABIN125988
  • Target See all Keratin Basic Antibodies
    Keratin Basic
    Reactivity
    • 21
    • 12
    • 10
    • 8
    • 7
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Cow, Amphibian
    Host
    • 13
    • 9
    Mouse
    Clonality
    • 22
    Monoclonal
    Conjugate
    • 14
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Un-conjugated
    Application
    • 19
    • 17
    • 16
    • 14
    • 10
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This antibody represents an excellent marker for distinguishing carcinomas from non-epithelial tumors. Polypeptides Reacting: Mr 52 500-Mr 68 000 keratins (type II keratins K1-K8, formerly also designated Cytokeratins 1-8) of Human epithelial cells. Tumors Specifically Detected: All epithelium-derived neoplasms. Tested Reactivities on Cultured Cell Lines: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, Bovine BMGE+H, BMGE-H, MDBK.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Bovine, Rat, Amphibia.
    Purification
    Affinity Chromatography on Protein A
    Immunogen
    Cytoskeletal proteins from cultured Human MCF-7 cells
    Clone
    Ks pan 1-8
    Isotype
    IgG2a
    Top Product
    Discover our top product Primary Antibody
  • Application Notes
    Immunoblotting. Immunofluorescence. Immunohistochemistry on frozen and cytological material: 1: 10, 1h at RT.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in 1 mL distilled water
    Buffer
    Final solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    This product is photosensitive and should be protected from light
    Storage
    4 °C
    Storage Comment
    Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • Target
    Keratin Basic
    Abstract
    Keratin Basic Products
    Background
    Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).
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