basic Cytokeratin antibody

Antigen: basic Cytokeratin

Clonality: Monoclonal (Ks pan 1-8)

Host: Mouse

Reactivity: Amphibian, Cow (Bovine), Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN125988

Additional Information

Alternative name:

Immunogen: Cytoskeletal proteins from cultured human MCF-7 cells

Cross-Reactivity: Amphibian, Cow (Bovine), Human

Isotype: IgG2a

Clone: Ks pan 1-8

Description: This antibody represents an excellent marker for distinguishing carcinomas fromnon-epithelial tumors.

Characteristics: Synonyms: HMW Cytokeratins, Type II Cytokeratins, Keratin Type II

Application Details

Application Notes: Immunoblotting. Immunofluorescence. Immunohistochemistry on frozen and cytological material: 1: 10, 1h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: PBS, pH7.4 containing 0.09 % sodium azide, 0.5% BSAReconstitution: Restore in 1 ml dist. water

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Gould, Koukoulis, Jansson et al.: "Coexpression patterns of vimentin and glial filament protein with cytokeratins in the normal, hyperplastic, and neoplastic breast." in: The American journal of pathology, Vol. 137, Issue 5, pp. 1143-55, 1990 (PubMed).

Heid, Moll, Franke: "Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles." in: Differentiation; research in biological diversity, Vol. 37, Issue 2, pp. 137-57, 1988 (PubMed).

Achtstaetter, Fouquet, Rungger-Braendle et al.: "Cytokeratin filaments and desmosomes in the epithelioid cells of the perineurial and arachnoidal sheaths of some vertebrate species." in: Differentiation; research in biological diversity, Vol. 40, Issue 2, pp. 129-49, 1989 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).