Integrin alpha-L (ITGAL) antibody

Details for Product No. ABIN126003
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Antigen
Synonyms (p180), Cd11a, LFA-1, LFA-1A, Ly-15, Ly-21, CD11A, LFA1A, CD11a
Reactivity
Human
(235), (182), (40), (23), (22), (22), (9), (5), (2), (1)
Host
Mouse
(268), (155), (35), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(83), (64), (53), (10), (5), (5), (5), (5), (4), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP)
(388), (112), (60), (59), (51), (45), (18), (18), (14), (12), (10), (8), (8), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 11 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN126003
308.00 $
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Immunogen Leucocytes from a pacient suffering from a LGL-type leukaemia
Clone MEM-25
Isotype IgG1
Specificity The antibody clone MEM-25 reacts with the alpha-subunit (CD11a) of human LFA-1, a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
Purification Protein A chromatography
Alternative Name CD11a / ITGAL
Background CD11a together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain
Gene ID 3683
NCBI Accession NP_002200
UniProt P20701
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry. Immunoprecipitation (excellent for immunoaffinity purification of LFA-1 complex)). Functional applications (MAb MEM-25 partially blocks binding of LFA-1 complex to ICAM-1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Ottonello, Epstein, Dapino et al.: "Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins." in: Blood, Vol. 93, Issue 10, pp. 3505-11, 1999 (PubMed).

Simon, Cherapanov, Nadra et al.: "Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2891-901, 1999 (PubMed).

Garnotel, Rittié, Poitevin et al.: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).

Hajishengallis, Martin, Sojar et al.: "Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 403-11, 2002 (PubMed).

Mathison, Befus, Davison et al.: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).

Sarantos, Raychaudhuri, Lum et al.: "Leukocyte function-associated antigen 1-mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule-1." in: The Journal of biological chemistry, Vol. 280, Issue 31, pp. 28290-8, 2005 (PubMed).

Giblin, Lemieux: "LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics." in: Current pharmaceutical design, Vol. 12, Issue 22, pp. 2771-95, 2006 (PubMed).

Kellersch, Kolanus: "Membrane-proximal signaling events in beta-2 integrin activation." in: Results and problems in cell differentiation, Vol. 43, pp. 245-57, 2006 (PubMed).

Fujii, Takada-Takatori, Kawashima: "Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity." in: Life sciences, Vol. 80, Issue 24-25, pp. 2320-4, 2007 (PubMed).

Wang, Kwas, Wu: "Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission." in: Journal of virology, Vol. 83, Issue 9, pp. 4195-204, 2009 (PubMed).

Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).

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