CD53 (CD53) antibody

Details for Product No. ABIN126005
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Antigen
Synonyms MOX44, TSPAN25, AI323659, Ox-44, Tspan25, OX44
Reactivity
Human
(72), (24), (16)
Host
Mouse
(70), (14), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(18), (8), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
(72), (33), (30), (29), (14), (10), (10), (7), (6), (5), (1)
Pubmed 12 references available
Catalog no. ABIN126005
Quantity 0.1 mg
Price
308.00 $   Plus shipping costs $45.00
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Immunogen Leukocytes of pacient suffering from a LGL-type leukemia
Clone MEM-53
Isotype IgG1
Specificity The antibody reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes, it is not present on platelets, red blood cells and non- hematopoietic cells. The antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Purification Protein-A affinity chromatography
Purity > 95% pure (by SDS-PAGE)
Alternative Name CD53
Background CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid- myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Alternate names: Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, TSPAN25, Tetraspanin-25
Gene ID 963
NCBI Accession NP_000551.1
UniProt P19397
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow cytometry. Immunoprecipitation. Western Blotting (non-reducing conditions). Immunohistochemistry on frozen tissue sections (suitable for discrimination of lymphomas from other tumors). Functional Application (MAb MEM-53 induces activation of monocytes and B lymphocytes). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Champault, Michel, Patel: "[Ulcers of the neck in hiatal hernias. Perforation into the free peritoneum (author's transl)]." in: La Nouvelle presse médicale, Vol. 7, Issue 33, pp. 2939-42, 1979 (PubMed).

Angelisová, Vlcek, Stefanová et al.: "The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens." in: Immunogenetics, Vol. 32, Issue 4, pp. 281-5, 1990 (PubMed).

Bazil, Stefanová, Hilgert et al.: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, 1990 (PubMed).

Rasmussen, Blomhoff, Stokke et al.: "Cross-linking of CD53 promotes activation of resting human B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 4997-5007, 1994 (PubMed).

Olweus, Lund-Johansen, Horejsi: "CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 2, pp. 707-16, 1993 (PubMed).

Szöllósi, Horejsí, Bene et al.: "Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2939-46, 1996 (PubMed).

Mollinedo, Martín-Martín, Gajate et al.: "Physiological activation of human neutrophils down-regulates CD53 cell surface antigen." in: Journal of leukocyte biology, Vol. 63, Issue 6, pp. 699-706, 1998 (PubMed).

Puls, Hogquist, Reilly et al.: "CD53, a thymocyte selection marker whose induction requires a lower affinity TCR-MHC interaction than CD69, but is up-regulated with slower kinetics." in: International immunology, Vol. 14, Issue 3, pp. 249-58, 2002 (PubMed).

Yunta, Lazo: "Apoptosis protection and survival signal by the CD53 tetraspanin antigen." in: Oncogene, Vol. 22, Issue 8, pp. 1219-24, 2003 (PubMed).

Yunta, Rodríguez-Barbero, Arévalo et al.: "Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primary cultures of mesangial cells." in: Kidney international, Vol. 63, Issue 2, pp. 534-42, 2003 (PubMed).

Kim, Yoon, Kim et al.: "LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress." in: Molecules and cells, Vol. 17, Issue 1, pp. 125-31, 2004 (PubMed).

General Szoellosi, Ferenc, Pinter et al.: "[Benign cystic mesothelioma, a rare tumor of the peritoneum]" in: Magyar sebészet, Vol. 58, Issue 1, pp. 35-7, 2005 (PubMed).

Hosts (70), (14), (5)
Reactivities (72), (24), (16)
Applications (72), (33), (30), (29), (14), (10), (10), (7), (6), (5), (1)
Conjugates (18), (8), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (2), (1)
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