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Solute Carrier Organic Anion Transporter Family, Member 1B1 (SLCO1B1) (N-Term) antibody

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(19), (16), (11), (8), (3), (2), (1), (1), (1)
(37), (2)
(34), (5)
Clonality (Clone)
Monoclonal ()
(2), (2), (2), (2), (2), (2)
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(37), (27), (4), (3), (3), (1), (1)
Pubmed 2 references available
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Quantity 5 mL
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic N-terminus (24 aa) of Human organic anion transporter OATP2 coupled to KLH
Clone MMDQ
Isotype IgG1
Specificity In Western blot analyses of basolateral membrane fractions of human liver the monoclonal antibody mMDQ reacts with 90 kDa and 120 kDa proteins (after SDS-PAGE) representing OATP2 (also described as OATP-C) and OATP8, respectively. According to a recent new nomenclature these hepatocyte-specific proteins are identical to OATP1B1 and OATP1B3, respectively (see Ref.2). Using immunofluorescence microscopy mMDQ stains the sinusoidal (basolateral) membranes in frozen sections of human liver, hepatocellular carcinoma (see Ref.1). Reacts with OATP2 and OATP8, human organic anion transporters (90 kDa, 120 kDa, after SDS-PAGE). Reacts with Transfected HEK293 cells, negative with HepG2 and PLC.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Also reacts to: OATP8
Purification Supernatant
Alternative Name SLCO1B1 / OATP2 (SLCO1B1 Antibody Abstract)
Background Synonyms: LST-1, LST1, Liver-specific organic anion transporter 1, OATP-2, OATP-C, OATPC, SLC21A6, Sodium-independent organic anion-transporting polypeptide 2, Solute carrier family 21 member 6, Solute carrier organic anion transporter family member 1B1
Gene ID 10599
NCBI Accession NP_006437
UniProt Q9Y6L6
Application Notes Immunoprecipitation. Immunofluorescence Microscopy. Western blotting: For optimal detection of the glycosylated antigen sample boiling beforeSDS-PAGE is not recommended. Alternatively, samples can be kept at 37 °C for 30 min. (SeeRef.1)Immunohistochemistry on Paraffin Sections (only after microwave treatment: useundiluted. For details See Ref.1). Incubation Time: 1 h at RT, for Paraffin Sections overnight at 2-8 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Western Blotting: (Ref. 1)1. For optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is notrecommended, alternatively, samples can be kept at 37°C for 30 min2. 50 µg of protein lysate should be loaded on each lane. 3. The usage of a PVDF-Membrane is recommended. 4. The usage of 10% milk powder in TTBS (Tris-buffered saline, pH 7. 6, with 0. 05% Tween20) as blocking reagent. The antibody can be diluted 3-5 x
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Cui, König, Nies et al.: "Detection of the human organic anion transporters SLC21A6 (OATP2) and SLC21A8 (OATP8) in liver and hepatocellular carcinoma." in: Laboratory investigation, a journal of technical methods and pathology, Vol. 83, Issue 4, pp. 527-38, 2003 (PubMed).

Hagenbuch, Meier: "The superfamily of organic anion transporting polypeptides." in: Biochimica et biophysica acta, Vol. 1609, Issue 1, pp. 1-18, 2002 (PubMed).

Catalog No. ABIN126013
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