Cytokeratin 1 (KRT1) antibody

Antigen: Cytokeratin 1 (KRT1)

Synonyms: K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK

Clonality: Polyclonal

Host: Guinea Pig

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN126064

Additional Information

Characteristics: Synonyms: Keratin-1, KRT1, K1, Cytokeratin 1, Hair alpha protein, 67 kDA cytokeratin, KRTA, Keratin typeII cytoskeletal 1

Alternative name: Cytokeratin 1

Swiss-Prot: P04264

Immunogen: Synthetic peptide of Human keratin K1 (formerly also designated Cytokeratin 1) coupled toKLHAA Sequence: SAGIINYQRRTTSS-C

Format: Serum

Description: Cytokeratin 1 is a type II Cytokeratin. The type II Cytokeratins consist of basic or neutralproteins which are arranged in pairs of heterotypic keratin chains coexpressed duringdifferentiation of simple and stratified epithelial tissues. This type II Cytokeratin isspecifically expressed in the spinous and granular layers of the epidermis with familymember KRT10 and mutations in these genes have been associated with bullouscongenital ichthyosiform erythroderma.

Specificity: Reacts with Basic keratin K1 (Mr 66 000). Specific for keratin K1 in suprabasal layers of cornified squamous epithelia, e. g. thesuprabasal layer of scalp and foot sole. Negative with non-keratinizing stratified and non-stratified epithelia (e. g. esophagus,duodenum)! Species Reactivity: Tested: Human and Mouse.

Application Details

Application Notes: Cytological Material. Western blotting: 1/3,000 (ECL). Immunohistochemistry on Frozen Sections: 1/200. Immunohistochemistry on Paraffin Embedded Material: 1/100 (after microwave treatmentor trypsin). Incubation Time: 1 h at RT, extended with paraffin sections. Positive Control: Human foot sole. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Storage: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Research Area: Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix

Restrictions: For Research Use only

Publications

Publications: Than, Sumegi, Than et al.: "Cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17): homology with adipophilin and the mouse adipose differentiation-related protein." in: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, Vol. 20, Issue 4, pp. 184-92, 1999 (PubMed).