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Keratin 7 (KRT7) antibody

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Antigen
  • CK7
  • D15Wsu77e
  • K2C7
  • K7
  • Krt2-7
  • KRT7
  • SCL
Reactivity
Human
477
133
65
8
7
6
5
5
3
2
1
1
1
Host
Guinea Pig
330
173
2
Clonality
Polyclonal
Conjugate
Un-conjugated
26
17
11
10
9
9
7
7
7
7
7
7
7
7
7
7
7
7
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
287
226
208
121
105
72
49
43
22
18
11
8
7
3
2
1
1
Supplier
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Immunogen Synthetic peptide (C-SAGPGLLKAYSIRT) of human keratin K7 (formerly also designated cytokeratin 7), coupled to KLH.
Specificity Reacts with basic keratin K7 (Mr 54 000). Excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas hepatocellular and prostate carcinomas are negative. Detects specific subtypes of adenocarcinomas: Adenocarcinoma of pancreas, gallbladder, lung, cervix, cholangio carcinoma of liver, ductal and lobular carcinoma of breast, carcinomas of ovary, transitional cell carcinoma of bladder, mesothelioma, negative with most cases of hepatocellular carcinoma. In colorectal carcinoma early stages are reported to be negative but advanced stages of tumor development are positive for keratin K7 expression.
Purification Serum
Alternative Name Cytokeratin 7 (KRT7 Antibody Abstract)
Background Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin
Gene ID 3855
NCBI Accession NP_005547
UniProt P08729
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes Western blotting: 1: 3000 (ECL). Immunohistochemistry on frozen (1: 200) and paraffin-embedded (1/100) sections (aftermicrowave treatment). Cytological material. Incubation time: 1 h at RT, extended with paraffin sections. Positive control: Glandular epithelia (e. g. sweat glands, sebaceous glands), Merkel cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
Expiry Date 6 months
Background publications Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983