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Keratin 8 (KRT8) antibody

Antigen

Keratin 8 (KRT8)
Synonyms
K8, KO, CK8, CYK8, K2C8, CARD2, Card2, EndoA, Krt2-8, Krt-2.8, AA960620, AL022697, AU019895, MGC118110, CYKER, KRT2-8, krt8, MGC53564, KRT8, ck8, cyk8, k2c8, card2, krt2-5, MGC69490, cb59, zf-K8, MGC7 ... show more
Clonality Polyclonal
Host
Alternatives

Guinea Pig
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)
1 reference available
Catalog no. ABIN126068
Quantity 0.1 ml
Price 495.00 $   Plus shipping costs $45.00
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Additional Information
Application Details Publications Alternatives back to top

Alternative name Cytokeratin 8
UniProt P05787
Immunogen Synthetic peptide (C-MSIRVTQKSYKVST) of human keratin K8 (formerly also designatedcytokeratin 8), coupled to KLH.
Cross-Reactivity Human, Mouse (Murine)
Description Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins andexists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the nonsquamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by theuse of antibodies that recognize only cytokeratin polypeptides 8 and 18.
Characteristics Synonyms: KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A, Keratin, type IIcytoskeletal 8
Specificity Specific for keratin K8. Marker for discrimination of simple / non-stratified epithelia fromthose of stratified origin. Basic human keratin K8 (Mr 52 000) of all simple type andcomplex epithelia. Species: Human, Mouse. Others not tested.
Synonyms K8, KO, CK8, CYK8, K2C8, CARD2, Card2, EndoA, Krt2-8, Krt-2.8, AA960620, AL022697, AU019895, MGC118110, CYKER, KRT2-8, krt8, MGC53564, KRT8, ck8, cyk8, k2c8, card2, krt2-5, MGC69490, cb59, zf-K8, MGC76893, wu:fa91a11, wu:fd19e09, wu:fl23c09, etID20321.6, zfk8, DreK8, cb186, krt2-8, MGC85764, sb:cb186, MGC174782, wu:fa20h05, wu:fa95h10, wu:fb96c06, DKFZp468F2127

Application Details
Publications Alternatives back to top

Application Notes Immunoblotting: 1: 3000, ECL method. Immunohistochemistry on frozen sections: 1: 200. Immunohistochemistry on paraffin sections: 1: 100, 1h at RT, after microwave treatment ortrypsin. Immunohistochemistry on cytological material. Positive Control: Human intestinal epitheliaOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Purification Serum
Storage Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
Research Area Cancer, Cytoskeleton, Cytokeratins, Extracellular Matrix
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Keratin 8 (KRT8)", type "Antibodies"
Hosts Mouse (104), Rabbit (96), Chicken (2), Goat (1), Guinea Pig (1)
Reactivities Human (180), Rat (Rattus) (46), Mouse (Murine) (44), Pig (Porcine) (27), Dog (Canine) (21), Chicken (20), Cow (Bovine) (12), Sheep (Ovine) (7), Zebrafish (Danio rerio) (4), Hamster (3), Rabbit (3), Xenopus laevis (2), Cat (Feline) (1)
Applications Western Blotting (WB) (126), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (94), Immunohistochemistry (IHC) (65), Immunofluorescence (IF) (59), ELISA (45), Immunocytochemistry (ICC) (33), Immunoprecipitation (IP) (31), Immunohistochemistry (Fixed) (IHC (fx)) (18), Flow Cytometry (FACS) (13), Immunohistochemistry (Frozen Sections) (IHC (fro)) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Immunoelectron Microscopy (IEM) (1), To be determined by user (TBD) (1)
Conjugates Biotin (11), FITC (3), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), HRP (2), PE,Cy5.5 (2), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes C-Term (6), pSer74 (6), pSer23 (5), pSer73 (4), AA 391-483 (3), AA 1-483 (2), Internal Region (2), N-Term (2), Ser74 (2), pSer431 (2), AA 353-367 (1), AA 450-477 (1), Center (1), Ser73 (1), pSer432 (1)