Keratin 13 (KRT13) antibody

Antigen: Keratin 13 (KRT13)

Synonyms: K13, CK13, MGC3781, MGC161462, Krt1-13, Krt-1.13, Ka13, k13, ck13, MGC84388, MGC115054, krt13, KRT15

Clonality: Polyclonal

Host: Guinea Pig

Reactivity: Human

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN126069

Additional Information

Alternative name: Cytokeratin 13

UniProt: P13646

Immunogen: Synthetic peptide (TTTSSASVTTTSNA-C) of human keratin K13 (formerly also designatedcytokeratin 13), coupled to KLH.

Cross-Reactivity: Human, Mouse (Murine)

Description: Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediatefilament proteins responsible for the structural integrity of epithelial cells and aresubdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist ofacidic proteins which are arranged in pairs of heterotypic keratin chains. This type Icytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornifiedstratified epithelia. Mutations in this gene and keratin 4 have been associated with theautosomal dominant disorder White Sponge Nevus.

Characteristics: Synonyms: CK-13, CK13, Keratin-13, KRT13, K13, KRT-13, Keratin type I cytoskeletal 13

Specificity: This antibody react to Acidic human keratin K13 (Mr 55 000). Excellent marker todiscriminate non-cornified squamous epithelia (esophagus, vagina, gingival) from those ofdifferent origin. Negative on foot sole, scalp. Tumors specifically detected: severalsquamous cell carcinomas, e. g. cervix carcinoma, transitional cell carcinoma of thebladder, craniopharyngiomaSpecies: Human, Mouse. Others not tested.

Application Details

Application Notes: Immunoblotting: 1: 3000, ECL method. Immunohistochemistry on frozen sections: 1: 200. Immunohistochemistry on paraffin sections: 1: 100, 1h at RT, after microwave treatment. Immunohistochemistry on cytological material. Positive Control: Squamous epithelium of esophagus, gingival, palate, vagina (staining allof the suprabasal layers). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Serum

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Research Area: Signaling, Cytoskeleton, Extracellular Matrix

Restrictions: For Research Use only

Publications

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).