Keratin 20 (KRT20) antibody

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Antigen
  • KRT20
  • CD20
  • CK-20
  • CK20
  • K20
  • KRT21
  • Krt21
  • 9030623C06Rik
  • keratin 20
  • keratin, type I cytoskeletal 20-like
  • KRT20
  • Krt20
  • LOC100520330
Reactivity
Human, Mouse (Murine)
293
65
53
18
8
3
2
1
1
Host
Guinea Pig
145
126
26
3
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
16
15
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
221
98
91
86
43
35
32
23
15
13
8
5
4
4
3
2
2
1
Options
Supplier
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Immunogen KLH conjugated synthetic peptide of Human keratin K20 (formerly also designated cytokeratin 20), coupled to KLH
Sequence KTT EYQ LST LEE RD-C
Specificity Reacts with Acidic Human Keratin K20 (Mr 46 000). Excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma).
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Purification Serum
Alternative Name Cytokeratin 20 (KRT20 Antibody Abstract)
Background Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
Gene ID 54474
NCBI Accession NP_061883
UniProt P35900
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer
Application Notes Western Blot: 1/3000 (ECL). Immunohistochemistry on Frozen Sections: 1/200. Immunohistochemistry on Paraffin Embedded Tissue: 1/100 (after microwave treatment ortrypsin). Cytological material. Incubation Time: 1 h at RT, extended with paraffin sections. Positive Control: Colon, duodenum, Merkel cells of skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).