Keratin 32 (KRT32) antibody

Details for Product No. ABIN126077
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Antigen
Synonyms Ka26, KRT32, krtha2, HA2, HKA2, KRTHA2, hHa2, Ha3, Hka2, K32, Krt1-2, Krtha2
Reactivity
Human
(5)
Host
Guinea Pig
(2), (2), (1)
Clonality
Polyclonal
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(5), (2), (1)
Pubmed 3 references available
Quantity 0.1 mL
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Immunogen Synthetic peptide of human hair (trichocytic) keratin K32 (formerly also designated keratin hHa2, CVTRTVCVPRTVGM), coupled to KLH
Specificity The antiserum stains specifically human hair keratin K32 expressed in the hair cuticle (Mr 45,000 polypeptide) and tumors derived from.
Purification Serum
Alternative Name Keratin-32 (KRT32)
Background Synonyms: HHA2, HKA2, Hair keratin, KRT-32, KRTHA2, Keratin, type I Ha2, type I cuticular Ha2
Gene ID 3882
NCBI Accession NP_002269
UniProt Q14532
Application Notes Immunoblotting: 1 : 2,000, ECL method. Immunohistochemistry on frozen sections: 1 : 200, 1h at RT. Immunohistochemistry on paraffin sections: 1 : 200, 1h at RT, after trypsin and microwavepretreatment. NOTE: For enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100(in PBS) for 1 - 5 min prior to first antibody incubation step.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Langbein, Schweizer: "Keratins of the human hair follicle." in: International review of cytology, Vol. 243, pp. 1-78, 2005 (PubMed).

Langbein, Spring, Rogers et al.: "Hair keratins and hair follicle-specific epithelial keratins." in: Methods in cell biology, Vol. 78, pp. 413-51, 2005 (PubMed).

Langbein, Rogers, Winter et al.: "The catalog of human hair keratins. I. Expression of the nine type I members in the hair follicle." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19874-84, 1999 (PubMed).

Validation Images
Catalog No. ABIN126077
456.50 $
Plus shipping costs $45.00
Quantity
Price
0.1 mL
456.50 $

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