Keratin 32 (KRT32) antibody

Antigen: Keratin 32 (KRT32)

Synonyms: HA2, HKA2, hHa2, KRTHA2, Ha3, mHa2, Krt1-2, MGC144672, MGC144673, Ka26, KRT32, krtha2

Clonality: Polyclonal

Host: Guinea Pig

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN126077

Additional Information

Alternative name: Keratin-32 (KRT32)

UniProt: Q14532

Immunogen: Synthetic peptide of human hair (trichocytic) keratin K32 (formerly also designated keratinhHa2, CVTRTVCVPRTVGM), coupled to KLH.

Cross-Reactivity: Human

Characteristics: Synonyms: KRT-32, HHA2, HKA2, KRTHA2, Hair keratin, type I Ha2, Keratin, type I cuticular Ha2

Application Details

Application Notes: Immunoblotting: 1 : 2,000, ECL method. Immunohistochemistry on frozen sections: 1 : 200, 1h at RT. Immunohistochemistry on paraffin sections: 1 : 200, 1h at RT, after trypsin and microwavepretreatment. NOTE: For enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100(in PBS) for 1 - 5 min prior to first antibody incubation step. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Serum

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Langbein, Rogers, Winter et al.: "The catalog of human hair keratins. I. Expression of the nine type I members in the hair follicle." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19874-84, 1999 (PubMed).

Langbein, Spring, Rogers et al.: "Hair keratins and hair follicle-specific epithelial keratins." in: Methods in cell biology, Vol. 78, pp. 413-51, 2005 (PubMed).

Langbein, Schweizer: "Keratins of the human hair follicle." in: International review of cytology, Vol. 243, pp. 1-78, 2005 (PubMed).