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Keratin 34 (KRT34) antibody

Details for Product No. ABIN126078
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Antigen
Synonyms Ka29, Krt1-4, HKA1, HA4, Ha-4, KRTHA4, hHa4, 4733401E01Rik, Ha2
Reactivity
Human, Mouse (Murine)
(14), (3), (2)
Host
Guinea Pig
(9), (4), (2)
Clonality
Polyclonal
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(15), (4), (4), (4), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 0.1 mL
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Catalog No. ABIN126078
500.50 $
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Immunogen Synthetic peptide of human acidic hair (trichocytic) keratin K34 (formerly also designated keratin hHa4, GPC GTS QKG CCN), coupled to KLH.
Specificity The antiserum stains specifically human hair keratin K34 expressed in the upper hair cortex cells. Reacts with human and mouse.
Purification Serum
Alternative Name Keratin-34 (KRT34)
Background KRT34 is a type I hair keratin. Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins are acidic proteins and are usually found as a heterotetramer with two molecules of a type II keratin.
Alternate names: HHA4, HKA4, Hair keratin, KRT-34, KRTHA4, Keratin, Keratin34, type I Ha4, type I cuticular Ha4
Gene ID 3885
NCBI Accession NP_066293.2
UniProt O76011
Application Notes Immunoblotting: 1 : 2,000, ECL method. Immunohistochemistry on frozen sections: 1/200, 1h at RT. NOTE: For enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100(in PBS) for 1 - 5 min prior to first antibody incubation step. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Langbein, Rogers, Winter et al.: "The catalog of human hair keratins. I. Expression of the nine type I members in the hair follicle." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19874-84, 1999 (PubMed).

Langbein, Spring, Rogers et al.: "Hair keratins and hair follicle-specific epithelial keratins." in: Methods in cell biology, Vol. 78, pp. 413-51, 2005 (PubMed).

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