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Keratin 35 (KRT35) antibody

Details for Product No. ABIN126079, Supplier: Log in to see
Antigen
  • AI662400
  • Ha-5
  • HA5
  • Ha5
  • hHa5
  • Keratin-35
  • Krt1-24
  • KRT35
  • KRTHA5
  • Krtha5
Reactivity
Human, Mouse (Murine)
24
4
1
1
1
1
1
1
1
1
1
Host
Guinea Pig
24
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
26
14
13
2
2
1
Supplier
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Immunogen KLH conjugated synthetic peptide of Human acidic hair (trichocytic) Keratin K35 (formerly also designated keratin hHa5.
Sequence YSS SPC KLP SLS PVA RS
Specificity Specific for Acidic Hair Keratin K35 (hHa5). The antiserum stains specifically Human hair keratin K35 expressed starting from the upper part of hair matrix, lower cortex, and hair cuticle.
Purification Serum
Alternative Name Keratin-35 (KRT35)
Background Synonyms: HHA5, HKA5, Hair keratin, K35, KRT35, KRTHA5, Keratin, Keratin 35, Keratin-35, Keratin35, type I Ha5, type I cuticular Ha5
Gene ID 3886
NCBI Accession NP_002271
UniProt Q92764
Application Notes Immunoblotting: 1 : 2,000, ECL method. Immunohistochemistry on frozen sections: 1 : 200, 1h at RT. NOTE: : For enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100(in PBS) for 1 - 5 min prior to first antibody incubation step.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for up to one month or at -20 °C for longer.
Background publications Langbein, Spring, Rogers, Praetzel, Schweizer: "Hair keratins and hair follicle-specific epithelial keratins." in: Methods in cell biology, Vol. 78, pp. 413-51, 2005

Langbein, Rogers, Winter, Praetzel, Beckhaus, Rackwitz, Schweizer: "The catalog of human hair keratins. I. Expression of the nine type I members in the hair follicle." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19874-84, 1999