Keratin 35 (KRT35) antibody

Details for Product No. ABIN126079
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Antigen
Synonyms HA5, Ha-5, hHa5, KRTHA5, Ha5, Krtha5, Krt1-24, AI662400, KRT35, Keratin-35
Reactivity
Human, Mouse (Murine)
(6), (2)
Host
Guinea Pig
(6), (1), (1)
Clonality
Polyclonal
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(8), (3), (2), (1), (1)
Pubmed 2 references available
Catalog no. ABIN126079
Quantity 0.1 mL
Price
500.50 $   Plus shipping costs $45.00
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Immunogen KLH conjugated synthetic peptide of Human acidic hair (trichocytic) Keratin K35 (formerly also designated keratin hHa5.
Sequence YSS SPC KLP SLS PVA RS
Specificity Specific for Acidic Hair Keratin K35 (hHa5). The antiserum stains specifically Human hair keratin K35 expressed starting from the upper part of hair matrix, lower cortex, and hair cuticle.
Purification Serum
Alternative Name Keratin-35 (KRT35)
Background Alternate names: HHA5, HKA5, Hair keratin, K35, KRT35, KRTHA5, Keratin, Keratin 35, Keratin-35, Keratin35, type I Ha5, type I cuticular Ha5
Gene ID 3886
NCBI Accession NP_002271.3
UniProt Q92764
Application Notes Immunoblotting: 1 : 2,000, ECL method. Immunohistochemistry on frozen sections: 1 : 200, 1h at RT. NOTE: : For enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100(in PBS) for 1 - 5 min prior to first antibody incubation step. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for up to one month or at -20°C for longer.
Expiry Date 12 months
Background publications Langbein, Rogers, Winter et al.: "The catalog of human hair keratins. I. Expression of the nine type I members in the hair follicle." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19874-84, 1999 (PubMed).

Langbein, Spring, Rogers et al.: "Hair keratins and hair follicle-specific epithelial keratins." in: Methods in cell biology, Vol. 78, pp. 413-51, 2005 (PubMed).

Hosts (6), (1), (1)
Reactivities (6), (2)
Applications (8), (3), (2), (1), (1)
Epitopes (3), (1), (1), (1)
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