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Keratin 37 (KRT37) antibody

Details for Product No. ABIN126081, Supplier: Login to see
Antigen
  • HA7
  • K37
  • KRTHA7
  • KRT37
Reactivity
Human, Monkey
2
1
Host
Guinea Pig
2
1
Clonality
Polyclonal
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
3
2
1
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Immunogen Synthetic peptide of human acidic hair (trichocytic) keratin K37 (formerly also designated keratin hHa7, CGP VTG GSP SGH GAS MGR), coupled to KLH.
Specificity The antiserum stains specifically Human hair keratin K37 expressed starting from the middle cortex of vellous hairs. It is negative in the anagen hair follicle.
Purification Serum
Alternative Name Keratin-37 (KRT37) (KRT37 Antibody Abstract)
Background Synonyms: HHA7, HKA7, Hair keratin, K37, KRT37, KRTHA7, Keratin, Keratin 37, Keratin-37, Keratin37, type I Ha7, type I cuticular Ha7
Gene ID 8688
NCBI Accession NP_003761
UniProt O76014
Pathways
Application Notes Immunoblotting: 1/2000 (ECL). Immunohistochemistry on frozen tissue: 1/200, 1h at RT. NOTE: For enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100(in PBS) for 1 - 5 min prior to first antibody incubation step.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Langbein, Spring, Rogers et al.: "Hair keratins and hair follicle-specific epithelial keratins." in: Methods in cell biology, Vol. 78, pp. 413-51, 2005 (PubMed).

Jave-Suarez, Langbein, Winter et al.: "Androgen regulation of the human hair follicle: the type I hair keratin hHa7 is a direct target gene in trichocytes." in: The Journal of investigative dermatology, Vol. 122, Issue 3, pp. 555-64, 2004 (PubMed).

Langbein, Rogers, Winter et al.: "The catalog of human hair keratins. I. Expression of the nine type I members in the hair follicle." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19874-84, 1999 (PubMed).