Keratin 38 (KRT38) antibody

Antigen: Keratin 38 (KRT38)

Synonyms: HA8, hHa8, KRTHA8, KRT37

Clonality: Polyclonal

Host: Guinea Pig

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN126082

Additional Information

Alternative name: Keratin-38 (KRT38)

UniProt: O76015

Immunogen: Synthetic peptide of human acidic hair (trichocytic) keratin K38 (formerly also designatedkeratin hHa8, TTC GPT CGA STT GSR F), coupled to KLH.

Cross-Reactivity: Human, Mouse (Murine)

Characteristics: Synonyms: KRT38, HHA8, HKA8, KRTHA8, Keratin-38, K38, Keratin38, Keratin 38, Keratin, type Icuticular Ha8, Hair keratin, type I Ha8

Specificity: The antiserum stains specifically human hair keratin K38 expressed starting from themiddle cortex. The staining in the middle cortex appears highly heterogeneous. Species: Human, Mouse. Others not tested.

Application Details

Application Notes: Immunoblotting: 1 : 2,000, ECL method. Immunohistochemistry on frozen sections: 1: 200, 1h at RT. NOTE: For enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100(in PBS) for 1 - 5 min prior to first antibody incubation step. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Serum

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Langbein, Rogers, Winter et al.: "The catalog of human hair keratins. I. Expression of the nine type I members in the hair follicle." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19874-84, 1999 (PubMed).

Langbein, Spring, Rogers et al.: "Hair keratins and hair follicle-specific epithelial keratins." in: Methods in cell biology, Vol. 78, pp. 413-51, 2005 (PubMed).