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KRT83 antibody (Keratin 83)

Details for Product anti-KRT83 Antibody No. ABIN126085, Supplier: Log in to see
Antigen
  • HB3
  • Hb-3
  • KRTHB3
  • Krt2-25
  • K83
  • KRT81
  • Keratin-83
Reactivity
Human, Mouse (Murine)
9
2
1
1
1
1
1
1
1
Host
Guinea Pig
6
2
2
Clonality
Polyclonal
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
10
2
2
Supplier
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Immunogen Synthetic peptide of human basic hair (trichocytic) keratin K83 (formerly also designated hHb3, C GFNSIGCGFRPGNF), coupled to KLH
Specificity The antiserum stains specifically human hair keratin K83 expressed starting from the middle cortex.
Purification Serum
Alternative Name Keratin-83 (KRT83) (KRT83 Antibody Abstract)
Background Synonyms: K2.10, K83, KRT83, KRTHB3, Keratin, Keratin 83, Keratin-83, Keratin83, Type II hair keratin Hb3, type II cuticular Hb3
Gene ID 3889
NCBI Accession NP_002273
UniProt P78385
Application Notes Immunoblotting: 1 : 2,000, ECL method. Immunohistochemistry on frozen sections: 1: 200, 1h at RT. NOTE: For enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100(in PBS) for 1 - 5 min prior to first antibody incubation step.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Langbein, Spring, Rogers et al.: "Hair keratins and hair follicle-specific epithelial keratins." in: Methods in cell biology, Vol. 78, pp. 413-51, 2005 (PubMed).

Langbein, Rogers, Winter et al.: "The catalog of human hair keratins. II. Expression of the six type II members in the hair follicle and the combined catalog of human type I and II keratins." in: The Journal of biological chemistry, Vol. 276, Issue 37, pp. 35123-32, 2001 (PubMed).