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Keratin 85 (KRT85) antibody

Details for Product No. ABIN126087, Supplier: Log in to see
  • AA589597
  • BB007925
  • ECTD4
  • Hb-5
  • Hb5
  • HB5
  • hHb5
  • Kb25
  • Krt2-18
  • KRTHB5
Human, Mouse (Murine)
Guinea Pig
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Immunogen Synthetic peptide of Human Basic Hair (trichocytic) Keratin K85 (formerly also designated hHb5, GRQ IST GPS AIG GSI TVV APD C), coupled to KLH.
Specificity The antiserum stains specifically Human hair keratin K85 expressed starting from the hair upper matrix, cortex and hair cuticle.
Cross-Reactivity (Details) Species reactivity (tested):Human and Mouse.
Purification Serum
Alternative Name Keratin-85 (KRT85) (KRT85 Antibody Abstract)
Background Synonyms: K12.12, K85, KRT85, KRTHB5, Keratin, Keratin 85, Keratin-85, Keratin85, Type II hair keratin Hb5, type II cuticular Hb5
Gene ID 3891
NCBI Accession NP_002274
UniProt P78386
Application Notes Immunohistochemistry on frozen sections: 1 : 200, 1h at RT. Immunoblotting: 1 : 2000, ECL method. NOTE: For enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100(in PBS) for 1 - 5 min prior to first antibody incubation step.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C or in small aliquots at -20 °C .
Background publications Langbein, Spring, Rogers, Praetzel, Schweizer: "Hair keratins and hair follicle-specific epithelial keratins." in: Methods in cell biology, Vol. 78, pp. 413-51, 2005 (PubMed).

Langbein, Rogers, Winter, Praetzel, Schweizer: "The catalog of human hair keratins. II. Expression of the six type II members in the hair follicle and the combined catalog of human type I and II keratins." in: The Journal of biological chemistry, Vol. 276, Issue 37, pp. 35123-32, 2001 (PubMed).