Keratin 86 (KRT86) antibody

Details for Product No. ABIN126088
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Antigen
Synonyms Kb26, KRT86, HB6, Hb1, KRTHB1, KRTHB6, MNX, hHb6, 5430421N21Rik, AI325212, Khb4, Krt2-10, Krt2-11, MHb4
Reactivity
Human, Mouse (Murine)
(12), (1)
Host
Guinea Pig
(9), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(13), (6), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mL
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Catalog No. ABIN126088
500.50 $
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Immunogen Synthetic peptide of human basic hair (trichocytic) keratin K86 (formerly also designated hHb6, APV VST RVS SVP SNS NVV VGT TNA C), coupled to KLH
Specificity The antiserum stains specifically hair keratin K86 expressed starting from the middle hair cortex. It can be defect in Monilethrix. Species Reactivity: Tested: Human and Mouse
Purification Serum
Alternative Name Keratin-86 (KRT86)
Background Alternate names: K2.11, K86, KRT86, KRTHB6, Keratin, Keratin 86, Keratin-86, Keratin86, Type II hair keratin Hb6, type II cuticular Hb6
Gene ID 3892
NCBI Accession NP_002275
UniProt O43790
Application Notes Immunoblotting: 1 : 2000, ECL method. Immunohistochemistry on frozen sections: 1 : 200, 1h at RT. NOTE: For enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100(in PBS) for 1 - 5 min prior to first antibody incubation step. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Langbein, Rogers, Winter et al.: "The catalog of human hair keratins. II. Expression of the six type II members in the hair follicle and the combined catalog of human type I and II keratins." in: The Journal of biological chemistry, Vol. 276, Issue 37, pp. 35123-32, 2001 (PubMed).

Langbein, Spring, Rogers et al.: "Hair keratins and hair follicle-specific epithelial keratins." in: Methods in cell biology, Vol. 78, pp. 413-51, 2005 (PubMed).

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