Synaptophysin (SYP) antibody

Details for Product No. ABIN126704
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Antigen
Synonyms syp-B, xsypii, MGC86267, MRXSYP, A230093K24Rik, AI848995, Syn, p38, Syp1, mrxsyp, syp, xsypi
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(174), (79), (52), (21), (3), (2), (2), (2), (1), (1), (1), (1)
Host
Mouse
(114), (108), (6), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(165), (89), (59), (36), (33), (27), (11), (9), (8), (4), (1), (1)
Pubmed 8 references available
Quantity 1 mL
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Catalog No. ABIN126704
379.50 $
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Immunogen Synaptophysin (presynaptic vesicles).
Clone SY38
Isotype IgG1
Specificity Reacts with Rat PC-12 cultured cell line. Polypeptide reacting: Mr 38 000 transmembrane glycoprotein of presynaptic vesicles. SY 38 represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas. Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid.
Purification Ascites
Alternative Name Synaptophysin / SYP
Background Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data), in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).
Alternate names: Major synaptic vesicle protein p38, Neuroendocrine Marker
Gene ID 6855
NCBI Accession NP_003170
UniProt P08247
Application Notes Immunoblotting. Immunocytochemistry. Immunohistochemistry on Frozen SectionsImmunohistochemistry on Paraffin Embedded Tissue (in No case proteolytic treatment). Working Dilutions: 1/10 for Immunohistochemistry. Incubation Time: 1h at RT, extended for paraffin sections (overnight at 2-8°C). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS 0.09% Sodium Azide 1% BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Product cited in: Khan, Tsigani, Rashid et al.: "Circulating tumor cells and EpCAM expression in neuroendocrine tumors." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 2, pp. 337-45, 2011 (PubMed).

Background publications Bennett, Calakos, Kreiner et al.: "Synaptic vesicle membrane proteins interact to form a multimeric complex." in: The Journal of cell biology, Vol. 116, Issue 3, pp. 761-75, 1992 (PubMed).

Wiedenmann, Franke, Kuhn et al.: "Synaptophysin: a marker protein for neuroendocrine cells and neoplasms." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 10, pp. 3500-4, 1986 (PubMed).

Gould, Lee, Wiedenmann et al.: "Synaptophysin: a novel marker for neurons, certain neuroendocrine cells, and their neoplasms." in: Human pathology, Vol. 17, Issue 10, pp. 979-83, 1986 (PubMed).

Gould, Wiedenmann, Lee et al.: "Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry." in: The American journal of pathology, Vol. 126, Issue 2, pp. 243-57, 1987 (PubMed).

Gould: "Synaptophysin. A new and promising pan-neuroendocrine marker." in: Archives of pathology & laboratory medicine, Vol. 111, Issue 9, pp. 791-4, 1987 (PubMed).

Wiedenmann, Franke: "Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles." in: Cell, Vol. 41, Issue 3, pp. 1017-28, 1985 (PubMed).

Leube: "The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains." in: Journal of cell science, Vol. 108 ( Pt 3), pp. 883-94, 1995 (PubMed).

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