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Synaptophysin (SYP) antibody
|Synonyms||Syn, p38, AI848995, A230093K24Rik, Syp1, syp-A, xsypi, MGC86277, syp-B, xsypii, MGC86267|
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|6 references available|
|Quantity||1 mL (Variants)|
|Price||363.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Alternative name||Synaptophysin / SYP|
|Immunogen||Synaptophysin (presynaptic vesicles).|
|Cross-Reactivity||Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)|
|Description||Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, representsa new class of actin-binding proteins which has first been localized in podocytes and asubset of telencephalic postsynaptic densities. In human tissue synaptopodin has amolecular weight of 73. 7 kD and pI of 9. 38 (calculated from sequence data), in mouse thecorresponding data are 74 kD, pI 9. 27. In SDS-PAGE the antigen appears as 100 kDpolypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributedto posttranslational modifications).|
|Characteristics||Synonyms: Neuroendocrine Marker, Major synaptic vesicle protein p38|
|Application Notes||Immunoblotting. Immunocytochemistry. Immunohistochemistry on Frozen SectionsImmunohistochemistry on Paraffin Embedded Tissue (in No case proteolytic treatment). Working Dilutions: 1/10 for Immunohistochemistry. Incubation Time: 1h at RT, extended for paraffin sections (overnight at 2-8°C). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS containing 1% BSA as stabilizer and 0.09% Sodium Azide aspreservative.|
|Storage||Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.|
|Restrictions||For Research Use only|
Bennett, Calakos, Kreiner et al.: "Synaptic vesicle membrane proteins interact to form a multimeric complex." in: The Journal of cell biology, Vol. 116, Issue 3, pp. 761-75, 1992 (PubMed).
Wiedenmann, Franke, Kuhn et al.: "Synaptophysin: a marker protein for neuroendocrine cells and neoplasms." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 10, pp. 3500-4, 1986 (PubMed).
Gould, Lee, Wiedenmann et al.: "Synaptophysin: a novel marker for neurons, certain neuroendocrine cells, and their neoplasms." in: Human pathology, Vol. 17, Issue 10, pp. 979-83, 1986 (PubMed).
Gould, Wiedenmann, Lee et al.: "Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry." in: The American journal of pathology, Vol. 126, Issue 2, pp. 243-57, 1987 (PubMed).
Gould: "Synaptophysin. A new and promising pan-neuroendocrine marker." in: Archives of pathology & laboratory medicine, Vol. 111, Issue 9, pp. 791-4, 1987 (PubMed).
Leube: "The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains." in: Journal of cell science, Vol. 108 ( Pt 3), pp. 883-94, 1995 (PubMed).
|Hosts||Rabbit (82), Mouse (62), Sheep (6)|
|Reactivities||Human (106), Rat (Rattus) (41), Mouse (Murine) (22), Cow (Bovine) (16), Pig (Porcine) (3), Chicken (2), Hamster (2), Monkey (2), Cat (Feline) (1), Guinea Pig (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (95), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (73), Immunohistochemistry (IHC) (41), ELISA (36), Immunohistochemistry (Fixed) (IHC (fx)) (21), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Immunofluorescence (IF) (12), Immunocytochemistry (ICC) (9), Flow Cytometry (FACS) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoprecipitation (IP) (4), Dot Blot (Dot) (1), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)) (1)|
|Conjugates||FITC (4), Biotin (3)|
|Epitopes||C-Term (12), N-Term (6), Internal Region (5), Tyr81 (2), pTyr81 (2), AA 245-258 (1), AA 41-62 (1), Center (1)|