Synaptophysin (SYP) antibody

Antigen: Synaptophysin (SYP)

Synonyms: Syn, p38, AI848995, A230093K24Rik, Syp1, syp-A, xsypi, MGC86277, syp-B, xsypii, MGC86267

Clonality: Monoclonal (SY38)

Host: Mouse

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN126704

Additional Information

Alternative name: Synaptophysin / SYP

UniProt: P08247

Immunogen: Synaptophysin (presynaptic vesicles).

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1

Clone: SY38

Description: Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, representsa new class of actin-binding proteins which has first been localized in podocytes and asubset of telencephalic postsynaptic densities. In human tissue synaptopodin has amolecular weight of 73. 7 kD and pI of 9. 38 (calculated from sequence data), in mouse thecorresponding data are 74 kD, pI 9. 27. In SDS-PAGE the antigen appears as 100 kDpolypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributedto posttranslational modifications).

Characteristics: Synonyms: Neuroendocrine Marker, Major synaptic vesicle protein p38

Application Details

Application Notes: Immunoblotting. Immunocytochemistry. Immunohistochemistry on Frozen SectionsImmunohistochemistry on Paraffin Embedded Tissue (in No case proteolytic treatment). Working Dilutions: 1/10 for Immunohistochemistry. Incubation Time: 1h at RT, extended for paraffin sections (overnight at 2-8°C). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Ascites

Buffer: PBS containing 1% BSA as stabilizer and 0.09% Sodium Azide aspreservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Bennett, Calakos, Kreiner et al.: "Synaptic vesicle membrane proteins interact to form a multimeric complex." in: The Journal of cell biology, Vol. 116, Issue 3, pp. 761-75, 1992 (PubMed).

Wiedenmann, Franke, Kuhn et al.: "Synaptophysin: a marker protein for neuroendocrine cells and neoplasms." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 10, pp. 3500-4, 1986 (PubMed).

Gould, Lee, Wiedenmann et al.: "Synaptophysin: a novel marker for neurons, certain neuroendocrine cells, and their neoplasms." in: Human pathology, Vol. 17, Issue 10, pp. 979-83, 1986 (PubMed).

Gould, Wiedenmann, Lee et al.: "Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry." in: The American journal of pathology, Vol. 126, Issue 2, pp. 243-57, 1987 (PubMed).

Gould: "Synaptophysin. A new and promising pan-neuroendocrine marker." in: Archives of pathology & laboratory medicine, Vol. 111, Issue 9, pp. 791-4, 1987 (PubMed).

Leube: "The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains." in: Journal of cell science, Vol. 108 ( Pt 3), pp. 883-94, 1995 (PubMed).