Phosphotyrosine antibody

Catalog No. ABIN126879

Product Details anti-Phosphotyrosine Antibody

Target: Phosphotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Polyclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Purified

Components: incl. positive Control

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: Positive Control for Immunonlot Applications. Phosphotyrosine Molecular Weight Marker:

Restrictions: For Research Use only

Handling

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Abstract: Phosphotyrosine Products

Target Type: Amino Acid

Background: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the nucleus. Phosphorylated epitopes may serve as docking sites for the assembley of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosin kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via the interaction of phosphotyrosine residues with SH2 or PTB domains. Antibodies direct against phosphorylated epitopes recognize the phosphorylated amino acid in the context of the surrounding amino acid sequence. Recognition is therefore dependent on 2 criteria: 1) phosphorylation and 2) the surrounding amino acid motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Since the amino acid sequence varies between different phosphorylation sites, certain proteins - though phosphorylated - may not be detected by the antibody. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity.