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SHC1 antibody (pTyr239, pTyr240)

SHC1 Reactivity: Human, Mouse, Dog WB, IHC (fro), EIA Host: Mouse Monoclonal 1-00E-003 unconjugated
Catalog No. ABIN126888
  • Target See all SHC1 Antibodies
    SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
    Binding Specificity
    • 28
    • 22
    • 20
    • 19
    • 12
    • 8
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr239, pTyr240
    Reactivity
    • 165
    • 124
    • 87
    • 9
    • 7
    • 7
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Dog
    Host
    • 152
    • 20
    Mouse
    Clonality
    • 151
    • 21
    Monoclonal
    Conjugate
    • 77
    • 10
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 1
    • 1
    This SHC1 antibody is un-conjugated
    Application
    • 147
    • 56
    • 38
    • 25
    • 18
    • 14
    • 14
    • 13
    • 13
    • 8
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody specifically recognizes shc when it is phosphorylated at Tyr 239/Tyr 240.
    Characteristics
    Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3,SHCA, Src homology 2 domain-containing-transforming protein C1
    Purification
    Size exclusion chromatography
    Components
    incl. positive Control
    Immunogen
    Synthetic phosphopeptide conjugated to KLH
    Clone
    1-00E-003
    Isotype
    IgG1
    Top Product
    Discover our top product SHC1 Primary Antibody
  • Application Notes
    Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunohistochemistry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1 mL H2O (15 min, RT).
    Buffer
    1 mLPBS / 0.09 % Na-azide / PEG and Sucrose
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C/-80 °C
    Storage Comment
    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
    Alternative Name
    SHC1 / SHC (SHC1 Products)
    Synonyms
    SHC1 antibody, SHC antibody, SHCA antibody, P66shc antibody, si:dkey-246i14.4 antibody, wu:fc10h03 antibody, shc antibody, p66shc antibody, Shc antibody, ShcA antibody, p66 antibody, SHC adaptor protein 1 antibody, Shc1p antibody, squalene--hopene cyclase antibody, SHC (Src homology 2 domain containing) transforming protein 1 antibody, SHC (Src homology 2 domain containing) transforming protein 1 L homeolog antibody, src homology 2 domain-containing transforming protein C1 antibody, SHC1 antibody, shc1 antibody, Shc1 antibody, shc1.L antibody
    Background
    Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signaling pathways.Synonyms: SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1
    Gene ID
    6464
    UniProt
    P29353
    Pathways
    RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2
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