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STAT3 antibody (pTyr705)

STAT3 Reactivity: Human, Mouse, Dog WB, IP, IF, IHC (fro), EIA Host: Mouse Monoclonal 9-00E-012 unconjugated
Catalog No. ABIN126898
  • Target See all STAT3 Antibodies
    STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
    Binding Specificity
    • 89
    • 54
    • 27
    • 8
    • 7
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr705
    Reactivity
    • 276
    • 142
    • 135
    • 38
    • 25
    • 25
    • 22
    • 17
    • 16
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Human, Mouse, Dog
    Host
    • 228
    • 57
    • 4
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 196
    • 95
    Monoclonal
    Conjugate
    • 163
    • 23
    • 17
    • 10
    • 9
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    This STAT3 antibody is un-conjugated
    Application
    • 217
    • 110
    • 75
    • 56
    • 51
    • 42
    • 41
    • 40
    • 27
    • 24
    • 14
    • 7
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Specificity
    The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1. AM00148PU-N specifically recognizes STAT3 phosphorylated at Tyr 705 in human, mouse and dog. The antibody does not crossreact with the non-phosphorylated form of STAT3 nor with unrelated tyrosine-phosphorylated proteins. AM00148PU-N is particularly suitable for Western blot and ELISA applications.
    Characteristics
    Synonyms: STAT-3, Acute-phase response factor, APRF, Signal transducer and activator oftranscription 3
    Purification
    Size exclusion chromatography
    Components
    incl. positive Control
    Immunogen
    Synthetic phosphopeptide conjuagted to KLH
    Clone
    9-00E-012
    Isotype
    IgG1
    Top Product
    Discover our top product STAT3 Primary Antibody
  • Application Notes
    Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10g per 106 pervanadate treated HepG2 cells. Immunocytochemistry: 1 - 10 μg/mL. Immunohistochemistry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1 mL H2O (15 min, RT).
    Buffer
    1 mL2 x PBS with 0.09 % Sodium Azide, PEG and Sucrose
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C/-80 °C
    Storage Comment
    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
    Alternative Name
    STAT3 (STAT3 Products)
    Synonyms
    1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody
    Background
    The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3
    Molecular Weight
    92 kDa
    Gene ID
    6774
    UniProt
    P40763
    Pathways
    JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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