VASP antibody (Vasodilator-Stimulated phosphoprotein) (pSer157)

Details for Product anti-VASP Antibody No. ABIN126908, Supplier: Log in to see
Antigen
  • vasp
  • MGC80889
  • wu:fb23b04
  • wu:fk84g05
  • zgc:110347
  • si:dkey-113g17.1
  • si:ch211-202c21.1
  • DDBDRAFT_0188463
  • DDBDRAFT_0229340
  • DDB_0188463
  • DDB_0229340
  • VASP
  • vasodilator-stimulated phosphoprotein
  • Vasodilator-stimulated phosphoprotein
  • zgc:162320
  • vasp
  • AaeL_AAEL010747
  • CpipJ_CPIJ004706
  • CpipJ_CPIJ004707
  • vasP
  • VASP
  • zgc:162320
  • Vasp
Alternatives
anti-Human VASP antibody for Enzyme Immunoassay
Epitope
pSer157
51
25
18
15
12
8
8
7
6
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
274
144
115
20
16
13
8
7
5
3
2
1
1
Host
Mouse
225
41
8
Clonality (Clone)
Monoclonal ()
Conjugate
This VASP antibody is un-conjugated
7
6
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
246
119
67
50
48
33
26
18
15
11
4
4
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Synthetic phosphopeptide conjugated to KLHEpitope: Phosphoserine 157 RRVpSNAG
Clone 5C6
Isotype IgG1
Specificity AM00155PU-N recognizes VASP only, when Ser 157 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA). The antibody does not cross-react with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, AM00155PU-N (clone 5C6) is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.
Characteristics Synonyms: Vasodilator-stimulated phosphoprotein
Purification Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
Alternative Name VASP (VASP Antibody Abstract)
Background VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines, paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation. Molecular Weight: 46/50 kDaSynonyms: Vasodilator-stimulated phosphoprotein
Gene ID 7408
UniProt P50552
Research Area Signaling, Extracellular Matrix
Pathways TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation
Application Notes Western blot ( 0.5 μg/mL for HRPO/ECL detection). Recommended: Casein/Tween 20 basedblocking and blot incubation buffer. ELISA (0.05 μg/mL). Immunoprecipitation (1 - 10 μg per 10^6 pervanadate-treated A431 cells). Immunocytochemistry (1 - 10 μg/mL, may tolerate 0.5 % formaldehyde fixation). Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer PBS with 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquot and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be storedat 2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles!
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibody (ABIN126908) Phosphospecificity: Whole cell extracts of control (co) or SNP treat...
Product cited in: Iyú, Glenn, White, Fox, Heptinstall: "Adenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 2, pp. 416-22, 2011 (PubMed).

Did you look for something else?