Low Density Lipoprotein Receptor-Related Protein 5/6 (LRP5/LRP6) antibody

Antigen: Low Density Lipoprotein Receptor-Related Protein 5/6 (LRP5/LRP6)

Clonality: Monoclonal (1A12)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN126948

Additional Information

Alternative name: LRP5 + LRP6

Immunogen: Synthetic peptide (DTGTDRIEVTR) derived from Human LRP-6. These residues arecompletely conserved between Human and Mouse and between LRP-5 and LRP-6.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG2b

Clone: 1A12

Description: LDL-related proteins, LRP-5 and LRP-6, are members of the LDL receptor superfamily, whichwere found to be involved in the canonical Wnt signaling pathway. Mammalian LRP-6 wasshown to bind to Wnt-1 and enhance Wnt-induced developmental processes in Xenopusembryos. Mice lacking LRP-6 exhibited developmental defects that are similar to thosecaused by deficiencies in various Wnt proteins. Recent work also shows that Wnt proteinsinduce the binding of LRP-5 to Axin, leading Axin degradation and β-catenin stabilization.

Characteristics: Synonyms: LRP5, LRP7, Low-density lipoprotein receptor-related protein 5, LRP6, Low-densitylipoprotein receptor-related protein 6

Specificity: Detects full length and a proteolytic fragment of LRP-6. Species: Human and Mouse. Others not tested.

Application Details

Application Notes: Western blot: 1-2 μg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: 10 mM Tris-glycine (pH 7.0) containing 0.15M NaCl, 25% Glycerol, 0.5%BSA, and 0.01% Sodium Azide

Storage: Store the antibody undiluted at -20°C or for long term storage (in aliquots) at -70°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Stem Cells, Extracellular Matrix, Wnt Signaling

Restrictions: For Research Use only

Publications

Li, Ambrosini, Chu et al.: in: (PubMed).