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Antibodies
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anti-Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) antibody
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) antibody
| Antigen |
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| Synonyms |
HME, MME, MGC138506, Mmel, AV378681, Mme, MMP12 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Flow Cytometry (FACS) (19), Western Blotting (WB) (19), Immunofluorescence (IF) (18), ELISA (8), Immunoprecipitation (IP) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (IHC) (5), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN127178 |
| Quantity |
0.1 mg (0.2 mg/ml) (Variants) |
| Price |
300.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Macrophage metalloelastase, HME, ME, Matrix metalloproteinase-12, MMP12, Macrophageelastase
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Alternative name
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MMP-12
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Swiss-Prot
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P39900
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Immunogen
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Synthetic peptide surrounding amino acid 460 of human MMP-12
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Description
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The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible forthe degradation of extracellular matrix components, including collagen, gelatin,fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentiallyactivated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophagemetalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 maycontribute to elastin degradation occurring in granulomatous skin diseases and may alsoparticipate in macrophage migration through the epidermal and vascular basementmembranes in inflammatory disorders.
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Specificity
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The antibody recognizes a ~50 kDa band from samples of human origin. Other species not tested.
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Application Notes
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Western Blot (1-4 μg/ml). Jurkat cell lysate can be used as a positive control. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.2 mg/ml
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Buffer
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PBS, pH 7.2, containing 0.1 % BSA and 0.02 % thimerosal
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Storage
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Store the antibody undiluted at (in aliquots) at -20°C to -70°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. RecommendedControl Peptides: AP00229CP-N
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use only
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Alternatives for antigen "Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12)", type "Antibodies"
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Hosts
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Rabbit (36), Goat (2), Mouse (2)
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Reactivities
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Human (37), Mouse (Murine) (23), Rat (Rattus) (20), Dog (Canine) (3), Chicken (2), Cow (Bovine) (2), Horse (Equine) (2), Pig (Porcine) (2), Rabbit (2), Guinea Pig (1)
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Applications
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Flow Cytometry (FACS) (19), Western Blotting (WB) (19), Immunofluorescence (IF) (18), ELISA (8), Immunoprecipitation (IP) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (IHC) (5), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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HRP (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (3), Cleaved-Glu106 (1)
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Alternatives 
