Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1) antibody

Antigen: Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1)

Synonyms: CARD4, NLRC1, CLR7.1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN127341

Additional Information

Alternative name: CARD4 / NOD1

UniProt: Q9Y239

Immunogen: A synthetic peptide corresponding to amino acids 494-512 of human Nod1/CARD4 AA Sequence:QLGFLRALPELGPGGDQQS

Cross-Reactivity: Human

Description: Innate immunity is present in all animals and is the common mode of defense against microorganisms. It detects microorganisms by specific proteins called pattern-recognition molecules (PRMs) (reviewed in Werts et al. 2006). There is a limited set of PRMs in each animal genome, and it has been postulated that the PRMs were evolutionarily selected to detect conserved components or motifs of microorganisms called pathogen-associated molecular patterns (PAMPs). PAMPs are found in a wide range of microorganisms and recognition of PAMPs by PRMs activates inflammatory signaling pathways, thereby stimulating an immune response. NOD proteins are a family of cytosolic proteins which have been implicated in innate recognition of bacteria, the induction of inflammatory responses, and the regulation of caspase activation and apoptosis. NOD1/CARD4 is a PRM that recognizes specific peptidoglycan (PGN) components of bacterial cell walls (reviewed in Strober et al. 2006, and Inohara et al. 2003). NOD1 is expressed by cell types that are exposed to PGN under physiological conditions including antigen-presenting cells (APCs) such as macrophages and dendritic cells, and epithelial cells (reviewed in Strober et al. 2006). NOD1 is thought to play a role in the pathogenesis of human gastrointestinal disease and is associated with inflammatory bowel diseases and asthma. It is involved in host defense against Helicobacter pylori infection of the gastric mucosa, a chronic infection that can lead to peptic ulcers and gastric cancer. Alternate Names: Nucleotide-binding oligomerization domain 1, Caspase-recruitment domain 4.

Application Details

Application Notes: Western blot analysis: 1:1000-1:2000 Immunohistochemistry on paraffin sections: 1:1000-1:5000 Immunohistochemistry on frozen sections. Immunoprecipitation: 1:50-1:200. Recommended Positive Control: Human colon. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: n/a Background: Innate immunity is present in all animals and is the common mode of defense against microorganisms. It detects microorganisms by specific proteins called pattern-recognition molecules (PRMs) (reviewed in Werts et al. 2006). There is a

Purification: Serum

Buffer: 0.05% sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling, Transcription Factors

Restrictions: For Research Use only

Images

Formalin-fixed, paraffin-embedded section of human sebaceous gland (A) and colon (B) stained for NOD1 expression using ABIN127341 at 1:2000. Hematoxylin counterstain. 10X magnification.

Publications

Strober, Murray, Kitani et al.: "Signalling pathways and molecular interactions of NOD1 and NOD2." in: Nature reviews. Immunology, Vol. 6, Issue 1, pp. 9-20, 2006 (PubMed).

Werts, Girardin, Philpott: "TIR, CARD and PYRIN: three domains for an antimicrobial triad." in: Cell death and differentiation, Vol. 13, Issue 5, pp. 798-815, 2006 (PubMed).