Thyroglobulin (TG) antibody

Antigen: Thyroglobulin (TG)

Synonyms: TGN, TDH3, AITD3, Tgn, cog, MGC117699, TG, cb717

Clonality: Monoclonal (2H11)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129506

Additional Information

Alternative name: Thyroglobulin

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with humanthyroglobulin protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells afterimmunization with thyroglobulin from human thyroid gland using conventional hybridoma technology.

Format: Liquid (sterile filtered)

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2H11

Description: Thyroglobulin (Tg) is synthesized by the follicular epithelial cells of the thyroid andsecreted from the thyroid gland with the stimulation of TSH and/or thyroid stimulating immunoglobulins.Thyroglobulin is a prognostic marker for Graves` disease. Thyroglobulin antibody has been useful in the positiveidentification of thyroid carcinomas of the papillary and follicular types.

Specificity: This protein A purified mouse monoclonal antibody reacts specifically with thyroglobulin inhuman tissues. The antibody recognizes a 330-kDa band corresponding to thyroglobulin. Cross reactivity withthyroglobulin from mouse, rat and dog will occur. Cross reactivity with thyroglobulin from other sources has notbeen determined.

Application Details

Application Notes: Recommended Dilutions: Generally a 1:50 to 1:200 dilution is suitable for most applications. A 30 to 60 minuteincubation time is usually sufficient. However, depending upon the fixation conditions and the staining systememployed, optimal incubation should be determined by the user. Both frozen sections and formalin fixed, paraffin. Note: This antibody is suitable for the detection of thyroglobulin in ELISA,immunohistochemistry, immunoblotting and immunoprecipitation. For immunohistochemistry, both frozen sectionsand formalin fixed, paraffin-embedded tissue sections can be used without epitope retrieval or enzyme digestion.This antibody is specific for the 330 kDa thyroglobulin protein. Thyroglobulin shows a cytoplasmic localization.Thyroid tissue can be used as a positive control.

Concentration: 1.6 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below forextended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standingat room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior toimmediate use. Expiration date is one (1) year from date of opening.

Research Area: Cancer, Hormones

Restrictions: For Research Use only

Images

Western blot using antibodies-online Immunochemical's Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 ug, right lane 3.0 ug) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti- Thyroglobulin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey