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ULP1 antibody

ULP1 Reactivity: Saccharomyces cerevisiae WB, ELISA, IP, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN129522
  • Target See all ULP1 products
    ULP1
    Reactivity
    Saccharomyces cerevisiae
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ULP-1 protein.
    Immunogentype:Recombinant
    Isotype
    IgG
  • Application Notes
    This purified polyclonal antibody reacts with yeast ULP-1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 72.4 kDa in size corresponding to yeast ULP-1 by western blotting in the appropriate lysate or extract.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with deionized water (or equivalent)
    Concentration
    5.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    ULP1
    Alternative Name
    ULP1
    Synonyms
    NIB1 antibody, SUMO protease ULP1 antibody, SUMO deconjugating enzyme Ulp1 antibody, ULP1 antibody, ulp1 antibody
    Background
    ULP-1, ubiquitin-like protein-specific protease 1, initially processes Smt3 and also acts as a deconjugating enzyme for Smt3 [Saccharomyces cerevisiae (Baker's yeast)].  Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression.  But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization.  Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein.  Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans.   Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3.  Three distinct steps can be distinguished in the SUMO modification pathway: 1) activation of SUMO, 2) transfer of SUMO to the conjugating enzyme, and 3) substrate modification. Since SUMO is synthesized as a precursor protein, a maturation step precedes the activation reaction. In yeast, C-terminal processing of the SUMO precursor is mediated by the processing protease Ulp1, which has an additional role in the deconjugation of SUMO-modified substrates. Mature SUMO is activated by SUMO-activating enzyme, an E1-like heterodimeric protein complex composed of Uba2 and Aos1.  Ulp1 function has provided evidence that SUMO modification in yeast, as has been suspected for vertebrates, plays an important role in nucleocytoplasmic trafficking.
    Synonyms: Probable sentrin specific protease antibody, Ubiquitin Like Protease antibody
    Gene ID
    856087, 6325237
    UniProt
    Q02724
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