Cyclin L1 (CCNL1) (isoform 1) antibody

Antigen: Cyclin L1 (CCNL1)

Synonyms: BM-001, PRO1073, ania-6a, Ccnl, AU018493, 2610030E23Rik, Ania-6a, MGC93069, fd99b09, zgc:55544, wu:fd99b09, MCM2, CCNL1, MGC132032, bm-001, pro1073, MGC108436, Ccnl1

Epitope: isoform 1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129528

Additional Information

Alternative name: Cyclin L1 (Isoform 1)

Gene ID: NP_064703

Swiss-Prot: Q6NVY9

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeatedimmunizations with a synthetic polypeptide corresponding to amino acids 314-369 of Human Cyclin L1 alpha protein.

Format: Liquid (sterile filtered)

Description: Cyclin L (also referred to as CCNL) isencoded by two highly related genes Cyclin L1 and CyclinL2 (CCNL1 and CCNL2, respectively). Cyclin L has beenshown to associate with the PITSLRE kinase and isinvolved in pre-mRNA processing. Both Cyclin L geneproducts can be alternatively spliced to produce twoisoforms (known as 1 and 2 or alpha and beta). Cyclin L2is ubiquitous, expressed at much higher levels thanCyclin L1, and thus is likely the major partner for theCDK11p110 protein kinase.

Specificity: The product was purified from monospecific antiserum by Protein A chromatography. Thisantibody is specific for human Cyclin L1 alpha and shows negligible reactivity to Cyclin L1 beta or Cyclin L2 isoforms. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for theimmunogen sequence. Cross reactivity with Cyclin L1 alpha protein from dog, rat, mouse and chicken is expected, as this sequence shows 87-96% homology with the protein from these sources. Cross reactivity with Cyclin L1 alpha homologues from other sources has not been determined. Protein Sequence: Human Cyclin L1 alpha , 526 aa, predicted MW 59.6 kDa 1 masgphstat aaaaassaap saggsssgtt tttttttggi ligdrlysev sltidhslip 61 eerlsptpsm qdgldlpset dlrilgceli qaagillrlp qvamatgqvl fhrffysksf 121 vkhsfeivam acinlaskie eaprrirdvi nvfhhlrqlr gkrtpsplil dqnyintknq 181 vikaerrvlk elgfcvhvkh phkiivmylq vlecernqtl vqtawnymnd slrtnvfvrf 241 qpetiacaci ylaaralqip lptrphwfll fgtteeeiqe icietlrlyt rkkpnyelle 301 kevekrkval qeaklkakgl npdgtpalst lggfspaskp ssprevkaee kspisinvkt 361 vkkepedrqq askspyngvr kdskrsrnsr sasrsrsrtr srsrshtprr hynnrrsrsg 421 tyssrsrsrs rshsesprrh hnhgsphlka khtrddlkss nrhghkrkks rsrsqsksrd 481 hsdaakkhrh erghhrdrre rsrsfershk skhhggsrsg hgrhrr

Application Details

Application Notes: This Protein A purified antibody hasbeen tested for use in ELISA, immunohistochemistrywestern blot, immunoprecipitation and immunofluores-cence. Specific conditions for reactivity should beoptimized by the end user. Expect a band ~ 59 kDa in sizecorresponding to Cyclin L1 alpha by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:5,000 - 1:25,000 WESTERN BLOT 1:2,000 - 1:5,000 IMMUNOHISTOCHEMISTRY 10 ug/ml - 20 ug/ml OTHER APPLICATIONS User Optimized

Concentration: 5.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration . Restore with 0.1 ml deionized water or equivalent. Diluteonly prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles offreezing and thawing. Expiration date is one (1) year from date of restoration.

Research Area: Cell Cycle

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Protein A Purified anti-Cyclin L1beta antibody shows detection of a band ~59 kDa corresponding to a Cyclin L1alpha (arrowhead) present in mouse brain whole cell lysate. Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:3,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye

Immunohistochemistry. antibodies-online's anti-Cyclin L1alpha antibody was used at a 10 ug/ml to detect Cyclin L1alpha in a variety of tissues including breast (collagen), heart, kidney (distal tubules), liver, skeletal muscle, ovary (granulosa and oocyte), pancreas (islet), placenta (trophoblast), prostate (epithelium), skin, spleen (endothelium), stomach (chief), testes (seminiferous epithelium and leydig), thymus (Hassal's corpuscle and lymphocytes) and uterus (glandular epithelium and stroma). Low to moderate background staining was noted. This image shows Cyclin L1alpha staining of human spleen. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.