Cyclin L1/L2 (CCNL1/2) (isoform 2) antibody

Antigen: Cyclin L1/L2 (CCNL1/2)

Epitope: isoform 2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129530

Additional Information

Alternative name: Cyclin L1/L2 (Isoform 2)

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeatedimmunizations with a synthetic construct consisting of full length Human Cyclin L1 beta protein.

Format: Liquid (sterile filtered)

Description: Cyclin L (also referred to as CCNL) isencoded by two highly related genes Cyclin L1 and CyclinL2 (CCNL1 and CCNL2, respectively). Cyclin L has beenshown to associate with the PITSLRE kinase and isinvolved in pre-mRNA processing. Both Cyclin L geneproducts can be alternatively spliced to produce twoisoforms (known as 1 and 2 or alpha and beta). Cyclin L2 isubiquitous, expressed at much higher levels than Cyclin L1,and thus is likely the major partner for the CDK11p110protein kinase.

Specificity: The product was purified from monospecific antiserum by Protein A chromatography. Thisantibody will react with all forms of Cyclin L beta (cyclin L1 beta and cyclin L2 beta ). Cross reactivity with Cyclin L beta proteins from other sources is unknown. Specific References: Loyera, P., Trembleya, J.H., Katonac, R., Kidd, V.J. and Lahtia, J.M. (2005) Role of CDK/cyclin complexes in transcription and RNA splicing.Cell. Signal. 17, 1033 to 1051. General

Application Details

Application Notes: Recommended Dilutions: ELISA 1:5,000 - 1:25,000 WESTERN BLOT 1:1,000 - 1:5,000 IMMUNOHISTOCHEMISTRY 2 ug/ml - 20 ug/ml OTHER APPLICATIONS User Optimized. NoteThis protein A purified antibody hasbeen tested for use in ELISA, western blot, immunohisto-chemistry, immunoprecipitation and immunofluorescence.Specific conditions for reactivity should be optimized bythe end user. Expect a band ~ 25 kDa to 35 kDa in sizecorresponding to Cyclin L1 beta and Cyclin L2 beta by western blotting in the appropriate cell lysate or extract.

Concentration: 5.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration . Restore with 0.1 ml deionized water or equivalent. Diluteonly prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles offreezing and thawing. Expiration date is one (1) year from date of restoration.

Research Area: Cell Cycle

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Protein A Purified anti-Cyclin L1/L2beta antibody shows detection of a band ~35 kDa corresponding to Cyclin L beta (arrowhead) present in mouse brain whole cell lysate (800 nm channel - green). Marker proteins appear red (700 nm channel) and were used for molecular weight comparisons. Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:2,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye

Immunohistochemistry. antibodies-online's Affinity Purified anti- Cyclin L1/L2 (isoform 2) antibody was used at a 10 ug/ml to detect cyclin L in a variety of tissues including breast, kidney, liver, lung, skeletal muscle, pancreas, prostate and spleen. In some tissues elevated background staining was noted. In these instances further optimization of dilution is suggested. This image shows Cyclin L staining of human skeletal muscle. Tissue was formalinfixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.