ADAM Metallopeptidase Domain 10 (ADAM10) antibody

Antigen: ADAM Metallopeptidase Domain 10 (ADAM10)

Synonyms: kuz, AD10, MADM, CD156c, HsT18717, kuzbanian, 1700031C13Rik, ADAM10, adam10, wu:fc03d12, ADAM-10, DKFZp459A157, LOC100219653, Adam10

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129538

Additional Information

Alternative name: ADAM10

Gene ID: NP_001101

Swiss-Prot: O14672

Immunogen: This IgG fraction antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human ADAM10 protein.

Format: Liquid (sterile filtered)

Description: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha ) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-alpha , notch, and its ligand delta, are all membrane-bound molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-alpha , notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ) and MADM, respectively (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Specificity: This IgG fraction antibody is directed against human ADAM10 protein. The product was purified from monospecific antiserum by DEAE chromatography. A BLAST analysis was used to suggest cross-reactivity with ADAM10 protein from human, bovine and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against ADAM10 from mouse based on a single amino acid change relative to this sequence. Reactivity against homologues from other sources is not known. Predicted Molecular Weight: Human ADAM10, MW 84.1 kDa (748 aa)

Application Details

Application Notes: This IgG fraction antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 84 k Da in size corresponding to ADAM10 protein by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:5,000 - 1:20,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY 1:250 - 1:1,000 OTHER APPLICATIONS User Optimized.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Proteolysis / Ubiquitin, Metalloprotease, Extracellular Matrix, Apoptosis/Necrosis, Alzheimer's Disease, Proteases

Restrictions: For Research Use only

Images

Western blot using antibodies-online's IgG fraction of anti-ADAM10 antibody shows detection of ADAM10 in Jurkat whole cell lysate in the absence (1) or presence (2) of blocking peptide. The membrane was probed with the primary antibody diluted to 1:1,000.

Immunohistochemistry using antibodies-online's IgG fraction of anti-ADAM10 antibody shows staining of ADAM10 in K562 cells. The cells were fixed with paraformaldehyde and reacted with primary antibody diluted to 1:500.