Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) antibody

Antigen: Interleukin-1 Receptor-Associated Kinase 1 (IRAK1)

Synonyms: IRAK, pelle, IRAK1, Plpk, mPLK, IRAK-1, Il1rak, IRAK1-S, AA408924, RGD1563841

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129539

Additional Information

Alternative name: IRAK (IL-1 Receptor-Associated Kinase)

Gene ID: NP_001560

Swiss-Prot: P51617

Immunogen: This IgG fraction antibody was prepared from whole rabbit serum produced by repeatedimmunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human IRAKprotein.

Format: Liquid (sterile filtered)

Description: IRAK (Interleukin-1 receptor-associatedkinase 1). Nuclear factor kappa B (NF- kappa B) is a ubiquitous transcription factor and an essential mediator of gene expressionduring activation of immune and inflammatory responses. NF- kappa B mediates the expression of a great variety of genes in response toextracellular stimuli including IL-1, TNF alpha and LPS. A serine/ threonine protein kinase associated with IL-1 receptor (IRAK) and itshomologue mouse pelle-like protein kinase (mPLK) were identifiedrecently (1,2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signalingmolecule to mediate IL-1 response (3). IRAK mediates a signalingcascade leading to NF- kappa B activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).

Specificity: This IgG fraction antibody is directed against human IRAK protein. The product waspurified from monospecific antiserum by DEAE chromatography. A BLAST analysis was used to suggest crossreactivity with IRAK protein from human, mouse and rat based on 100% homology with the immunizing sequence.No cross reactivity occurs against IRAK-2. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: This IgG fractionantibody has been tested for use in ELISA,immunoprecipitation, immunohistochemistryand western blot. Specific conditions forreactivity should be optimized by the end user.Expect a band approximately 80 kDa in sizecorresponding to IRAK protein by westernblotting in the appropriate cell lysate or extract. ELISA 1:5,000 - 1:20,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY 1:100 - 1:500 IMMUNOPRECIPITATION 2-4 u g per sample OTHER APPLICATIONS User Optimized

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute onlyprior to immediate use. For extended storage aliquot contents andfreeze at -20° C or below. Avoid cycles of freezing and thawing.Expiration date is one (1) year from date of opening.

Research Area: Cytokines, Receptors, Inflammation

Restrictions: For Research Use only

Images

Western blot using antibodies-online's IgG fraction of anti-IRAK antibody shows detection of IRAK in THP whole cell lysate (1) or HeLa whole cell lysate (2). The membrane was probed with the primary antibody diluted to 1:2,000.