Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A) antibody

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A)

Synonyms: DR4, APO2, CD261, MGC9365, TRAILR1, TRAILR-1, TNFRSF10A

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129540

Additional Information

Alternative name: Death Receptor 4 (DR4)

Gene ID: NP_003835

Swiss-Prot: O00220

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human DR4 protein.

Format: Liquid (sterile filtered)

Description: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines in the TNF family, including TNF and Fas ligand, through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4) (1). The ligand for this novel death receptor has been identified and termed TRAIL (2,3), which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1) (4). DR4 is expressed in most human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF- kappa B activation in many tissues and cells.

Specificity: This antibody is directed against human DR4 protein. The product was purified from monospecific antiserum by DEAE ion exchange chromatography. The antibody is human, mouse and rat reactive. Reactivity against homologues from other sources has not been determined.

Application Details

Application Notes: Recommended Dilutions: ELISA User Optimized WESTERN BLOT 0.5-1 ug/ml IMMUNOCYTOCHEMISTRY 10 ug/ml OTHER APPLICATIONS User Optimized. Note: This antibody has been tested for use in western blotting and immunohistochemistry. HeLa whole cell lysate can be used as a positive control for western blotting, and a band at approximately 57 kDa is expected. Specific conditions for reactivity should be optimized by the end user.

Concentration: 0.5 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Restrictions: For Research Use only

Images

Western blot analysis of DR4 in HeLa total cell lysate with DR4 antibody at 1 ug/ml.

Immunocytochemistry of DR4 in Jurkat cells with DR4 antibody at 10 ug/ml.