SARS-CoV Ucleocapsid Protein (N-Term) antibody

Antigen: SARS-CoV Ucleocapsid Protein

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunofluorescence (IF), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129544

Additional Information

Alternative name: SARS-CoV Nucleocapsid (N) Protein

Swiss-Prot: P59595

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a purified recombinant protein corresponding to full length SARS Coronavirus Nucleocapsid protein. Lifesensors Inc.( www.lifesensors.com ) prepared the Nucleocapsid protein as follows: SUMO-Nucleocapsid fusion was expressed in E.coli in LB medium and purified using using Ni-NTA resin (Qiagen) affinity chromatography. After the fusion was cleaved by theSUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the nucleocapsid using MAC and thenucleocapsid was finally purified using Cation Exchange Chromatography with the Macro-Prep High S resin (BioRad) and sizeexclusion chromatography.

Format: Liquid (sterile filtered)

Description: The coronavirusnucleocapsid protein is the major structuralcomponent of virions that associates with genomicRNA to form a long, flexible, helical nucleocapsid.Sequence comparison of the N genes of five strainsof the coronavirus mouse hepatitis virus suggests athree-domain structure for the nucleocapsid protein. The nucleocapsid protein may be associated withcellular membranes where it participates in viral RNAsynthesis and virus budding.

Specificity: This protein A purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein. Theproduct was purified from monospecific antiserum by protein A affinity purification. BLAST analysis was used to suggestreactivity with related Coronavirus proteins. Cross reactivity with homologues from other sources has not been determined.

Application Details

Application Notes: This protein A purified antibody has been tested for use in ELISA and by western blot. Specificconditions for reactivity should be optimized by the end user. Expect a band approximately 46 kDa in size corresponding toSARS Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:10,000 - 1:50,000 WESTERN BLOT 1:2,000 - 1:10,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 5.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior torestoration. Reconstitute in 0.1 ml of deionized wateror equivalent. Dilute only prior to immediate use. Forextended storage aliquot contents and freeze at -20° Cor below. Avoid cycles of freezing and thawing.Expiration date is one (1) year from date of opening.

Research Area: Virology

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46-kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDye