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Application Notes
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This affinity-purified antibody is suitable for use in ELISA, western blotting and immunoprecipitation assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
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Concentration
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1.0 mg/ml
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Buffer
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Stabilizer: 50% glycerol. Preservative: None. Buffer: 0.02 M KPhosphate, 0.15 M NaCl, pH 7.2.
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Storage
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Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
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Research Area
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Amino Acids, Protein Modifications
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Restrictions
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For Research Use only
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