SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A-Like 1 (SMARCAL1) antibody

Antigen: SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A-Like 1 (SMARCAL1)

Synonyms: HARP, HHARP, Mharp, 6030401P21Rik, SMARCAL1, smarcal1, im:7145331, harp, MGC115661, hharp, MGC145606

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129604

Additional Information

Alternative name: SmarcAL1

Gene ID: NP_003003

Swiss-Prot: Q9NZC9

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a synthetic peptide corresponding aa 80-96 of human SmarcAL1 protein. Protein Sequence: Human SmarcAL1, 954 aa, predicted MW 105.9 kDa 1 mslplteeqr kkieenrqka larraeklla eqhqrtssgt siagnpfqak qgpsqnfpre 61 sckpvshgvi fkqqnlssss nadqrphdsh sfqakgiwkk peemptacpg hsprsqmalt 121 gispplaqsp pevpkqqlls yelgqghaqa speirftpfa npthkplakp kssqetpahs 181 sgqpprdakl eaktakasps gqnisyihss sesvtprteg rlqqksgssv qkgvnsqkgk 241 cvrngdrfqv ligynaelia vfktlpskny dpdtktwnfs mndysalmka aqslptvnlq 301 plewaygsse spstssegqa glpsapslsf vkgrcmlisr ayfeadisys qdlialfkqm 361 dsrrydvktr kwsflleehs kliakvrclp qvqldplptt ltlafasqlk ktslsltpdv 421 peadlsevdp klvsnlmpfq ragvnfaiak ggrllladdm glgktiqaic iaafyrkewp 481 llvvvpssvr ftweqaflrw lpslspdcin vvvtgkdrlt aglinivsfd llsklekqlk 541 tpfkvviide shflknsrta rcraampvlk vakrvillsg tpamsrpael ytqiiavkpt 601 ffpqfhafgl rycdakrmpw gwdysgssnl gelkllleea vmlrrlksdv lsqlpakqrk 661 ivviapgrin artraaldaa akemttkdkt kqqqkdalil ffnrtaeaki psvieyildl 721 lesgrekflv fahhkvvlda itqelerkhv qhiridgsts saeredlcqq fqlserhava 781 vlsitaanmg ltfssadlvv faelfwnpgv liqaedrvhr igqtssvgih ylvakgtadd 841 ylwpliqeki kvlaeaglse tnfsemtest dylykdpkqq kiydlfqksf ekegsdmell 901 eaaesfdpgs asgtsgsssq nmgdtldess ltaspqkkrr feffdnwdsf tspl

Format: Liquid (sterile filtered)

Description: SmarcAL1 (SWI/SNF-relatedmatrix-associated actin-dependent regulator ofchromatin a-like 1) is also known as HARP,HepA Related Protein, HHARP, SMARCA likeProtein 1 and SWI/SNF Related. SmarcAL1 is amember of the SWI/SNF family of proteins.Members of this family have helicase andATPase activities and are thought to regulatetranscription of certain genes by altering thechromatin structure around those genes. Theencoded protein shows sequence similarity tothe E. coli RNA polymerase-binding proteinHepA. Mutations in this gene are a cause ofSchimke immunoosseous dysplasia (SIOD), anautosomal recessive disorder with the diagnosticfeatures of spondyloepiphyseal dysplasia, renaldysfunction, and T-cell immunodeficiency.

Specificity: This affinity purified antibody is directed against human SmarcAL1 protein. The product wasaffinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggestreactivity with this protein from human based on 100% homology for the immunogen sequence. Cross reactivity with SmarcAL1 from other sources is not expected due to significant sequence divergence within the immunogen sequence.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 106 kDa in sizecorresponding to SmarcAL1 by western blotting in the appropriate cell lysate or extract.

Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Research Area: Chromatin and Nuclear Signaling

Restrictions: For Research Use only

Images

Western blot using antibodies-online's Affinity Purified anti-SmarcAL1 antibody shows detection of a band ~106 kDa band (arrowhead) corresponding to SmarcAL1 in human derived cultured cell lysates (HeLa nuclear extract lane 1, HeLa lane 2, A431 lane 3, Jurkat lane 4, and 293 lane 5). Approximately 5 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SmarcAL1 antibody. Signal was detected using standard techniques. SmarcAL1 is the band seen between the 97 and 116kD markers.