MCM2 antibody (AA 21-31)

Catalog No. ABIN129678

Product Details anti-MCM2 Antibody

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Binding Specificity: AA 21-31

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Yeast (Saccharomyces cerevisiae)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 21-31 of human MCM2 protein (see below).

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 100 kDa in size corresponding to phosphorylated or unphosphorylated MCM2 proteins by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.98 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: MCM2 (MCM2 Products)

Synonyms: CG7538 antibody, DmMCM2 antibody, DmMcm2 antibody, DmeMCM2 antibody, Dmel\\CG7538 antibody, MCM2 antibody, MCM2-7 antibody, MCM2_DROME antibody, McM2 antibody, PCR3 antibody, dMCM2 antibody, l(3)rL074 antibody, XMcm2 antibody, bm28 antibody, ccnl1 antibody, cdc19 antibody, cdcl1 antibody, mitotin antibody, cb737 antibody, chunp6905 antibody, BM28 antibody, CCNL1 antibody, CDCL1 antibody, D3S3194 antibody, MITOTIN antibody, MCM7 antibody, AA959861 antibody, AW476101 antibody, Mcmd2 antibody, mKIAA0030 antibody, Minichromosome maintenance 2 antibody, minichromosome maintenance complex component 2 antibody, minichromosome maintenance complex component 2 L homeolog antibody, Mcm2 antibody, mcm2 antibody, MCM2 antibody, mcm2.L antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. MCM2, also called DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog or Nuclear Protein BM28) is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by protein kinases CDC2 and CDC7.  Double serines at positions 26 and 27 are conserved in rat and mouse.  High homology >88% to similar proteins from several other species.  Expected broad reactivity to various lysates.
Synonyms: BM28 antibody, CCNL 1 antibody, CCNL1 antibody, CDC like 1 antibody, Minichromosome maintenance complex component 2 antibody

Gene ID: 4171, 33356547

UniProt: P49736

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA