Mitogen-Activated Protein Kinase 3 (MAPK3) antibody

Antigen: Mitogen-Activated Protein Kinase 3 (MAPK3)

Synonyms: ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180, zERK1, fi06b09, wu:fi06b09, Tb08.10J17.940

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129696

Additional Information

Alternative name: p44 ERK1

Gene ID: NP_002737

Swiss-Prot: P27361

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p44 ERK1 protein. Protein Sequence: Human p44 ERK1, 379 aa, predicted MW 43.1 kDa

Format: Liquid (sterile filtered)

Description: Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK's) ERK1 and ERK2. When activated, ERK's form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Specificity: This affinity-purified antibody is directed against the human p44 ERK1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with p44 ERK1 proteins from human, bovine, rabbit, rat and mouse. No reactivity is observed against p42 ERK2. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 45 kDa in size corresponding to p44 ERK1 by western blotting in the appropriate cell lysate or extract.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.1 M Tris HCl, 0.15 M Sodium Chloride, pH 7.5.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody