Myeloid Differentiation Primary Response Gene (88) (MYD88) antibody

Antigen: Myeloid Differentiation Primary Response Gene (88) (MYD88)

Synonyms: MYD88D, XMyD88, myd88d, MGC84928, DMMYD88, EP(2)2535, Kra, LD20892, MYD88, dMyD88, DmelCG2078, CG2078, zgc:103541, GB12344, NV10640, myd88, IRAK-2, MGC150550, AI649099, MGC102586, 6330415L08Rik, irak-2, MGC79591, IRAK2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129704

Additional Information

Alternative name: MyD88 (Myeloid Differentiation Primary Response Protein MyD88)

Swiss-Prot: Q99836

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human MyD88 protein.

Format: Liquid (sterile filtered)

Description: MyD88 (Myeloid differentiation primary response protein). The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway (1). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and the dominant negative form of MyD88 attenuates IL-1R-mediated NF- kappaΒ activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (2,3), both members of the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to the bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. The MyD88 gene is expressed in many tissues.

Specificity: This affinity purified antibody is directed against human MyD88 protein. The product was purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with MyD88 protein from human and mouse sources based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Synonyms: MYD88D, XMyD88, myd88d, MGC84928, DMMYD88, EP(2)2535, Kra, LD20892, MYD88, dMyD88, DmelCG2078, CG2078, zgc:103541, GB12344, NV10640, myd88, IRAK-2, MGC150550, AI649099, MGC102586, 6330415L08Rik, irak-2, MGC79591, IRAK2

Application Details

Application Notes: This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 33 kDa in size corresponding to MyD88 protein by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:5,000 - 1:20,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 0.5 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.02% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

anti-Myeloid Differentiation Primary Response Gene (88) (MYD88) antibody