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CD40 Ligand antibody (FITC)

CD40LG Reactivity: Mouse FACS Host: Armenian Hamster Monoclonal MR-1 FITC
Catalog No. ABIN1302930
  • Target See all CD40 Ligand (CD40LG) Antibodies
    CD40 Ligand (CD40LG)
    Reactivity
    • 138
    • 75
    • 36
    • 9
    • 9
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 87
    • 62
    • 21
    • 14
    • 11
    • 1
    Armenian Hamster
    Clonality
    • 108
    • 82
    • 4
    Monoclonal
    Conjugate
    • 102
    • 21
    • 20
    • 15
    • 9
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD40 Ligand antibody is conjugated to FITC
    Application
    • 105
    • 101
    • 54
    • 48
    • 38
    • 31
    • 20
    • 16
    • 15
    • 14
    • 13
    • 13
    • 7
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    The Armenian hamster monoclonal antibody MR-1 detects an extracellular epitope on murine CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
    Cross-Reactivity (Details)
    Mouse
    Purification
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    Activated mouse Th1 clone D1.6
    Clone
    MR-1
    Isotype
    IgG1
    Top Product
    Discover our top product CD40LG Primary Antibody
  • Application Notes
    Flow cytometry: Recommended dilution: 2-3 μg/mL, positive control: 6-8 hour activated murine splenocytes.
    Comment

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Shin, Kennedy, Gorski, Tsuchiya, Koseki, Azuma, Yagita, Chen, Powell, Pardoll, Housseau: "Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor." in: The Journal of experimental medicine, Vol. 198, Issue 1, pp. 31-8, (2003) (PubMed).

    Luzina, Atamas, Storrer, daSilva, Kelsoe, Papadimitriou, Handwerger: "Spontaneous formation of germinal centers in autoimmune mice." in: Journal of leukocyte biology, Vol. 70, Issue 4, pp. 578-84, (2001) (PubMed).

    Hwang, Nahm, Briles, Thomas, Purkerson: "Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L." in: Infection and immunity, Vol. 68, Issue 2, pp. 511-7, (2000) (PubMed).

    Lode, Xiang, Pertl, Förster, Schoenberger, Gillies, Reisfeld: "Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction." in: The Journal of clinical investigation, Vol. 105, Issue 11, pp. 1623-30, (2000) (PubMed).

    Howard, Miga, Vanderlugt, Dal Canto, Laman, Noelle, Miller: "Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis." in: The Journal of clinical investigation, Vol. 103, Issue 2, pp. 281-90, (1999) (PubMed).

    Salgado, Nakamura, Sugaya, Tada, Asahina, Koyama, Irie, Tamaki: "Functional CD40 ligand is expressed on epidermal Langerhans cells." in: Journal of leukocyte biology, Vol. 66, Issue 2, pp. 281-5, (1999) (PubMed).

    Wu, Zhou, Zheng, Liu: "CD28-independent induction of T helper cells and immunoglobulin class switches requires costimulation by the heat-stable antigen." in: The Journal of experimental medicine, Vol. 187, Issue 7, pp. 1151-6, (1998) (PubMed).

    Noelle, Roy, Shepherd, Stamenkovic, Ledbetter, Aruffo: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, (1992) (PubMed).

  • Target
    CD40 Ligand (CD40LG)
    Alternative Name
    CD154 / CD40L (CD40LG Products)
    Synonyms
    CD154 antibody, cd40l antibody, CD40L antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TNFSF5 antibody, TRAP antibody, gp39 antibody, hCD40L antibody, CD40-L antibody, Cd40l antibody, Ly-62 antibody, Ly62 antibody, Tnfsf5 antibody, CD40 ligand antibody, TNF superfamily member 5 antibody, CD40LG antibody, cd40l antibody, Cd40lg antibody
    Background
    CD40 ligand,CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.,CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM
    Gene ID
    959
    UniProt
    P29965
    Pathways
    NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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